Hepatitis B virus proteins expressed by recombinant vaccinia viruses : influence of preS2 sequence on expression surface and nucleocapsid proteins in human diploid cells

• Published in: Archives of virology 1994-01, Vol.134 (1-2), p.1-15
• Main Authors: KUTINOVA, L, NEMECKOVA, S, LOPAREV, V, VONKA, V, HAMSIKOVA, E, ZAVADOVA, H, LUDVIKOVA, V, BROUCEK, J, KUNKE, D, KÖNIG, J, ZAKHAROVA, L. G, PASHVYKINA, G. V
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Viral - biosynthesis; Biological and medical sciences; Cell Line; Chick Embryo; Cloning, Molecular; Female; Fundamental and applied biological sciences. Psychology; Haplorhini; Hepatitis B Antigens - biosynthesis; Hepatitis B Antigens - genetics; Hepatitis B Antigens - immunology; Hepatitis B Surface Antigens - biosynthesis; Hepatitis B Surface Antigens - genetics; Hepatitis B Surface Antigens - immunology; Humans; Mice; Mice, Inbred ICR; Microbiology; Protein Precursors - biosynthesis; Protein Precursors - genetics; Protein Precursors - immunology; Protein Sorting Signals - genetics; Protein Sorting Signals - physiology; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccinia virus - genetics; Vaccinia virus - physiology; Virology
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/BF01379102

Fifteen vaccinia virus (VV) recombinants derived from VV strains Praha, LIVP and DD (i.e. Dryvax Wyeth vaccine-derived) and expressing genes for S, preS2-S or c antigens of hepatitis B virus (HBV) were tested in monkey CV-1 cells and human diploid LEP cells. The production of infectious virus was found to be alike in all the recombinants and parental viruses as well. However, several recombinants produced markedly lesser amounts of S and preS2 antigens in LEP cells than in CV-1 cells. This reduction was independent of the parental virus used. There was, however, a relationship between the production of preS2 in CV-1 cells and the production of S and preS2 antigens in LEP cells; in general, recombinants efficiently inducing preS2 antigen formation in CV-1 cells produced markedly reduced amounts of S and preS2 antigens in LEP cells. Reduction of HBV antigen production in LEP cells was not apparent in recombinants expressing only S or c antigens of HBV, and the production of c antigen by double recombinants was not influenced by simultaneous expression of preS2 and S. The various recombinants also differed in the ratio of S:preS2 antigen formation. This difference seemed to be associated with the length of the untranslated leader sequence preceding preS2 but not with the parental virus or cell type used. The titers of antibodies against S and preS2 antigens induced in mice immunized with different recombinants differed markedly. The differences in the ratio of S:preS2 antigen production in vitro were not reflected in vivo by S:preS2 antibody ratio.