Platelet Calcium Transport In Hypertension

To determine platelet Ca transport entities involved in increased cytosolic Ca in the platelets of hypertensive individuals, we studied the relations between blood pressure and Ca transporters in platelet membranes from 22 white male volunteers 32 to 68 years old. We used thapsigargin, a specific in...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 1994-01, Vol.23 (1), p.31-37
Main Authors: Dean, William L, Pope, Jennie E, Brier, Michael E, Aronoff, George R
Format: Article
Language:English
Subjects:
Adult
Aged
Arterial hypertension. Arterial hypotension
Biological and medical sciences
Blood and lymphatic vessels
Blood Platelets - metabolism
Calcium - metabolism
Calcium-Transporting ATPases - blood
Cardiology. Vascular system
Clinical manifestations. Epidemiology. Investigative techniques. Etiology
Humans
Hypertension - blood
Hypertension - metabolism
Ion Transport
Male
Medical sciences
Middle Aged
Platelet Membrane Glycoproteins - analysis
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Summary:To determine platelet Ca transport entities involved in increased cytosolic Ca in the platelets of hypertensive individuals, we studied the relations between blood pressure and Ca transporters in platelet membranes from 22 white male volunteers 32 to 68 years old. We used thapsigargin, a specific inhibitor of the internal membrane Ca-ATPase, to differentiate between plasma membrane and internal membrane Ca-ATPases. Inositol 1,4,5-trisphosphate-mediated and Ca ionophore (A23187)-induced Ca release was also assayed in membrane preparations using rhod-2, a fluorescent Ca indicator. Levels of glycoprotein Illa, a possible component of agonist-mediated Ca influx, were measured by immunoblotting. The results show that plasma membrane Ca- ATPase is decreased as a function of diastolic blood pressure (P
ISSN:0194-911X
1524-4563
DOI:10.1161/01.HYP.23.1.31