Expression cloning of an insect diuretic hormone receptor: a member of the calcitonin/secretin receptor family

Insect diuretic hormones and their receptors regulate fluid and ion secretion and thus are attractive targets for the design of novel insect control agents. A complementary DNA clone encoding a corticotropin-releasing factor-related diuretic hormone receptor from the tobacco hornworm Manduca sexta w...

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-01, Vol.269 (1), p.9-12
Main Author: Reagan, J.D
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Cell Line
Cell receptors
Cell structures and functions
CLONACION
CLONAGE
Cloning, Molecular
CORTICOTROPHINE
CORTICOTROPINA
DNA, Complementary
Fundamental and applied biological sciences. Psychology
HORMONAS DE INVERTEBRADOS
HORMONE D'INVERTEBRE
Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors
Insect Hormones - metabolism
Insect Proteins
MANDUCA SEXTA
Molecular and cellular biology
Molecular Sequence Data
Moths
RECEPTEUR D'HORMONE
RECEPTORES DE HORMONAS
Receptors, Calcitonin - genetics
Receptors, Cell Surface
Receptors, G-Protein-Coupled
Receptors, Gastrointestinal Hormone - genetics
Receptors, Gastrointestinal Hormone - metabolism
Receptors, Peptide - genetics
Secretin - metabolism
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
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Summary:Insect diuretic hormones and their receptors regulate fluid and ion secretion and thus are attractive targets for the design of novel insect control agents. A complementary DNA clone encoding a corticotropin-releasing factor-related diuretic hormone receptor from the tobacco hornworm Manduca sexta was isolated by expression cloning in COS-7 cells. The receptor consists of 395 amino acids and contains seven putative transmembrane domains. The expressed receptor binds M. sexta diuretic hormone, as well as several related insect diuretic peptides with high affinity. Furthermore, each of these peptides stimulate adenylate cyclase in COS-7 cells transfected with the receptor. The M. sexta diuretic hormone receptor is homologous to the receptors for calcitonin, secretion, vasoactive intestinal peptide, parathyroid hormone, glucagon-like peptide 1, growth hormone-releasing hormone, pituitary adenylate cyclase-activating polypeptide, and glucagon. The M. sexta diuretic hormone receptor is the first nonmammalian member of this family to be identified
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(17)42299-x