Tubulointerstitial nephritis antigen interacts with laminin and type IV collagen and promotes cell adhesion

Tubulointerstitial nephritis (TIN) antigen has been recently identified as a novel basement membrane macromolecule. It consists of a single chain of 58 kDa and exhibits a restricted distribution. The interaction between TIN antigen and laminin or type IV collagen has been studied using solid-phase b...

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-01, Vol.269 (3), p.1654-1659
Main Authors: Kalfa, T A, Thull, J D, Butkowski, R J, Charonis, A S
Format: Article
Language:English
Subjects:
Animals
Basement Membrane - metabolism
Cattle
Cell Adhesion - drug effects
Cell Adhesion Molecules - isolation & purification
Cell Adhesion Molecules - metabolism
Cell Adhesion Molecules - pharmacology
Cells, Cultured
Collagen - metabolism
Endothelium, Vascular - drug effects
Endothelium, Vascular - physiology
Epithelium - drug effects
Epithelium - physiology
Heparin - metabolism
Kidney Cortex - metabolism
Kidney Tubules - drug effects
Kidney Tubules - physiology
Kinetics
Laminin - metabolism
Membrane Glycoproteins - isolation & purification
Membrane Glycoproteins - metabolism
Membrane Glycoproteins - pharmacology
Mice
Nephelometry and Turbidimetry
Rabbits
Telomere-Binding Proteins
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Summary:Tubulointerstitial nephritis (TIN) antigen has been recently identified as a novel basement membrane macromolecule. It consists of a single chain of 58 kDa and exhibits a restricted distribution. The interaction between TIN antigen and laminin or type IV collagen has been studied using solid-phase binding assays and found to be for both macromolecules specific, saturable, and with an affinity in the low micromolar range. In similar assays, TIN antigen did not interact with heparin. In turbidimetry assays, it was found that the presence of TIN antigen did not affect the polymerization of type IV collagen but had a concentration-dependent inhibitory effect on laminin polymerization and on preformed laminin polymers. TIN antigen was able to promote adhesion of epithelial cells derived from kidney tubules and of endothelial cells derived from aorta. The data suggest that TIN antigen may be a macromolecule of importance both for basement membrane ultrastructure and cellular adhesion.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)42077-1