In vitro Proliferation and Production of Gamma Interferon by Murine CD4+Cells in Response to Cryptosporidium parvum Antigen

Spleen cells from mice immunized with Cryptosporidium parvum were enriched for T cells by passage over an affinity chromatography column. The proliferative response of these cells was >2-fold higher than the response of unenriched cells. T-enriched cells were enriched further for either CD4+cells...

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Bibliographic Details
Published in:The Journal of parasitology 1994-02, Vol.80 (1), p.67-72
Main Authors: Harp, James A., Whitmire, William M., Sacco, Randy
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens, Protozoan - immunology
Biological and medical sciences
CD4-Positive T-Lymphocytes - immunology
Cryptosporidium parvum - immunology
Cultured cells
Cytokines
Experimental protozoal diseases and models
Female
Harps
Immunity
Immunology
Infections
Infectious diseases
Interferon-gamma - biosynthesis
Interferons
Lymphocyte Activation
Medical sciences
Mice
Mice, Inbred BALB C
Parasitic diseases
Protozoal diseases
Spleen - cytology
Spleen - immunology
Splenocytes
T lymphocytes
T-Lymphocyte Subsets - immunology
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Summary:Spleen cells from mice immunized with Cryptosporidium parvum were enriched for T cells by passage over an affinity chromatography column. The proliferative response of these cells was >2-fold higher than the response of unenriched cells. T-enriched cells were enriched further for either CD4+cells or CD8+cells. The proliferative response of enriched cells was >4-fold higher than the response by unenriched cells. CD8+cells were essentially nonresponsive to C. parvum antigen. Culture supernatant fractions from these variously enriched splenocyte populations were assayed for cytokine production. Cultures containing CD4+cells produced gamma interferon and interleukin-2 following incubation with C. parvum antigen. None of the cultures produced interleukin-4. Production of gamma interferon and interleukin-2, but not interleukin-4, is characteristic of the previously described Th1 helper cell subset. Our data indicate that a subset of murine lymphocytes consistent with the Th1 helper cell phenotype proliferates following in vitro stimulation with C. parvum antigen.
ISSN:0022-3395
1937-2345
DOI:10.2307/3283347