A novel gene delivery system using EGF receptor-mediated endocytosis

A monoclonal antibody to the human epidermal growth factor (EGF) receptor was conjugated with polylysine and the resulting conjugate was affinity-linked with DNA (gene). This novel gene delivery system utilizes receptor-mediated endocytosis and would be especially suitable for gene therapy for EGF r...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1994-01, Vol.338 (2), p.167-169
Main Authors: Chen, Jiabing, Gamou, Shinobu, Takayanagi, Atsushi, Shimizu, Nobuyoshi
Format: Article
Language:English
Subjects:
3-acethyl chloramphenicol
Antibodies, Monoclonal
Biological and medical sciences
Biotechnology
Carcinoma, Squamous Cell
CAT, chloramphenicol acetyltransferase
Chloramphenicol O-Acetyltransferase - genetics
CM, chloramphenicol
DNA - administration & dosage
DNA - metabolism
EBSS, Earle's balanced salt solution
EGF receptor
EGF, epidermal growth factor
Electrophoresis, Agar Gel
Endocytosis
ErbB Receptors - physiology
Fundamental and applied biological sciences. Psychology
Gene therapy
Gene Transfer Techniques
Health. Pharmaceutical industry
Humans
Industrial applications and implications. Economical aspects
MoAb, monoclonal antibody
Monoclonal antibody
Polylysine
SPDP, N-succinimidyl 3-(2-pyridyldithio) propionate
TNF, tumor necrosis factor, 3-AcCM
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A monoclonal antibody to the human epidermal growth factor (EGF) receptor was conjugated with polylysine and the resulting conjugate was affinity-linked with DNA (gene). This novel gene delivery system utilizes receptor-mediated endocytosis and would be especially suitable for gene therapy for EGF receptor-overproducing squamous cell carcinomas.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)80357-9