Loading…
Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis
Folic acid was covalently conjugated to 66-nm liposomes via spacers of various lengths in an attempt to target the liposomes to KB cells expressing folate receptors. Spacers of short and intermediate lengths were unable to mediate association of folate-conjugated liposomes with receptor-bearing cell...
Saved in:
| Published in: | The Journal of biological chemistry 1994-02, Vol.269 (5), p.3198-3204 |
|---|---|
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c438t-ac7f45cc390f97bc0effee13227bbefb703661a08ae0ed26f2e4e8ba664a89fd3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c438t-ac7f45cc390f97bc0effee13227bbefb703661a08ae0ed26f2e4e8ba664a89fd3 |
| container_end_page | 3204 |
| container_issue | 5 |
| container_start_page | 3198 |
| container_title | The Journal of biological chemistry |
| container_volume | 269 |
| creator | LEE, R. J LOW, P. S |
| description | Folic acid was covalently conjugated to 66-nm liposomes via spacers of various lengths in an attempt to target the liposomes
to KB cells expressing folate receptors. Spacers of short and intermediate lengths were unable to mediate association of folate-conjugated
liposomes with receptor-bearing cells, however, use of a 250 A polyethyleneglycol spacer (PEG, M(r) approximately 3350) permitted
avid uptake of the liposomes at approximately 2.5 x 10(5) sites/cell. The binding of folate-PEG liposomes to KB cells could
be competitively inhibited by excess free folate or by antiserum against the folate receptor, demonstrating the interaction
is mediated by the cell surface folate-binding protein. Following binding, cell-associated folate-PEG liposomes were internalized
by folate-receptor-mediated endocytosis at 37 degrees C but not at 4 degrees C. These folate-PEG liposomes show potential
for delivering large quantities of low molecular weight compounds nondestructively into folate receptor-bearing cells. |
| doi_str_mv | 10.1016/S0021-9258(17)41848-5 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76358288</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16818699</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-ac7f45cc390f97bc0effee13227bbefb703661a08ae0ed26f2e4e8ba664a89fd3</originalsourceid><addsrcrecordid>eNqFkEtP3DAURi1EBcPjJyBZFaroIuAbO469bKdPFYkFILGzHOeacZWMBzuhmn9PhhnNtt5Y8nfuw4eQC2DXwEDe3DNWQqHLSl1B_VmAEqqoDsgMmOIFr-DpkMz2yDE5yfkvm47QcESOFDDJKzEjj9-wC6-Y1jR62oVVzLHHTMNyiNSN3TAmbOmfr9Rh12X6Giz1sbMD0oQOV0NMRY9tmB5aiss2uvUQc8hn5IO3Xcbz3X1KHn98f5j_Km7vfv6ef7ktnOBqKKyrvaic45p5XTeOofeIwMuybhr0Tc24lGCZssiwLaUvUaBqrJTCKu1bfko-bfuuUnwZMQ-mD3mzql1iHLOpp0-qUqn_giAVKKn1BFZb0KWYc0JvVin0Nq0NMLPxbt69m41UA7V5926qqe5iN2BsJiP7qp3oKb_c5TY72_lkly7kPSYYA8E3bT5usUV4XvwLCU0Toltgb0qpTWU4aMXfAMvWl8I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16818699</pqid></control><display><type>article</type><title>Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis</title><source>ScienceDirect (Online service)</source><creator>LEE, R. J ; LOW, P. S</creator><creatorcontrib>LEE, R. J ; LOW, P. S</creatorcontrib><description>Folic acid was covalently conjugated to 66-nm liposomes via spacers of various lengths in an attempt to target the liposomes
to KB cells expressing folate receptors. Spacers of short and intermediate lengths were unable to mediate association of folate-conjugated
liposomes with receptor-bearing cells, however, use of a 250 A polyethyleneglycol spacer (PEG, M(r) approximately 3350) permitted
avid uptake of the liposomes at approximately 2.5 x 10(5) sites/cell. The binding of folate-PEG liposomes to KB cells could
be competitively inhibited by excess free folate or by antiserum against the folate receptor, demonstrating the interaction
is mediated by the cell surface folate-binding protein. Following binding, cell-associated folate-PEG liposomes were internalized
by folate-receptor-mediated endocytosis at 37 degrees C but not at 4 degrees C. These folate-PEG liposomes show potential
for delivering large quantities of low molecular weight compounds nondestructively into folate receptor-bearing cells.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)41848-5</identifier><identifier>PMID: 8106354</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Biological and medical sciences ; Biological Transport - drug effects ; Carrier Proteins - metabolism ; Diverse techniques ; Drug Carriers ; Endocytosis - drug effects ; Folate Receptors, GPI-Anchored ; Folic Acid - analogs & derivatives ; Folic Acid - pharmacology ; Fundamental and applied biological sciences. Psychology ; Humans ; Immune Sera - pharmacology ; KB Cells ; Kinetics ; Liposomes ; Molecular and cellular biology ; Polyethylene Glycols ; Receptors, Cell Surface</subject><ispartof>The Journal of biological chemistry, 1994-02, Vol.269 (5), p.3198-3204</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-ac7f45cc390f97bc0effee13227bbefb703661a08ae0ed26f2e4e8ba664a89fd3</citedby><cites>FETCH-LOGICAL-c438t-ac7f45cc390f97bc0effee13227bbefb703661a08ae0ed26f2e4e8ba664a89fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4001435$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8106354$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LEE, R. J</creatorcontrib><creatorcontrib>LOW, P. S</creatorcontrib><title>Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Folic acid was covalently conjugated to 66-nm liposomes via spacers of various lengths in an attempt to target the liposomes
to KB cells expressing folate receptors. Spacers of short and intermediate lengths were unable to mediate association of folate-conjugated
liposomes with receptor-bearing cells, however, use of a 250 A polyethyleneglycol spacer (PEG, M(r) approximately 3350) permitted
avid uptake of the liposomes at approximately 2.5 x 10(5) sites/cell. The binding of folate-PEG liposomes to KB cells could
be competitively inhibited by excess free folate or by antiserum against the folate receptor, demonstrating the interaction
is mediated by the cell surface folate-binding protein. Following binding, cell-associated folate-PEG liposomes were internalized
by folate-receptor-mediated endocytosis at 37 degrees C but not at 4 degrees C. These folate-PEG liposomes show potential
for delivering large quantities of low molecular weight compounds nondestructively into folate receptor-bearing cells.</description><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Carrier Proteins - metabolism</subject><subject>Diverse techniques</subject><subject>Drug Carriers</subject><subject>Endocytosis - drug effects</subject><subject>Folate Receptors, GPI-Anchored</subject><subject>Folic Acid - analogs & derivatives</subject><subject>Folic Acid - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immune Sera - pharmacology</subject><subject>KB Cells</subject><subject>Kinetics</subject><subject>Liposomes</subject><subject>Molecular and cellular biology</subject><subject>Polyethylene Glycols</subject><subject>Receptors, Cell Surface</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNqFkEtP3DAURi1EBcPjJyBZFaroIuAbO469bKdPFYkFILGzHOeacZWMBzuhmn9PhhnNtt5Y8nfuw4eQC2DXwEDe3DNWQqHLSl1B_VmAEqqoDsgMmOIFr-DpkMz2yDE5yfkvm47QcESOFDDJKzEjj9-wC6-Y1jR62oVVzLHHTMNyiNSN3TAmbOmfr9Rh12X6Giz1sbMD0oQOV0NMRY9tmB5aiss2uvUQc8hn5IO3Xcbz3X1KHn98f5j_Km7vfv6ef7ktnOBqKKyrvaic45p5XTeOofeIwMuybhr0Tc24lGCZssiwLaUvUaBqrJTCKu1bfko-bfuuUnwZMQ-mD3mzql1iHLOpp0-qUqn_giAVKKn1BFZb0KWYc0JvVin0Nq0NMLPxbt69m41UA7V5926qqe5iN2BsJiP7qp3oKb_c5TY72_lkly7kPSYYA8E3bT5usUV4XvwLCU0Toltgb0qpTWU4aMXfAMvWl8I</recordid><startdate>19940204</startdate><enddate>19940204</enddate><creator>LEE, R. J</creator><creator>LOW, P. S</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940204</creationdate><title>Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis</title><author>LEE, R. J ; LOW, P. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-ac7f45cc390f97bc0effee13227bbefb703661a08ae0ed26f2e4e8ba664a89fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Carrier Proteins - metabolism</topic><topic>Diverse techniques</topic><topic>Drug Carriers</topic><topic>Endocytosis - drug effects</topic><topic>Folate Receptors, GPI-Anchored</topic><topic>Folic Acid - analogs & derivatives</topic><topic>Folic Acid - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immune Sera - pharmacology</topic><topic>KB Cells</topic><topic>Kinetics</topic><topic>Liposomes</topic><topic>Molecular and cellular biology</topic><topic>Polyethylene Glycols</topic><topic>Receptors, Cell Surface</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LEE, R. J</creatorcontrib><creatorcontrib>LOW, P. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LEE, R. J</au><au>LOW, P. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-02-04</date><risdate>1994</risdate><volume>269</volume><issue>5</issue><spage>3198</spage><epage>3204</epage><pages>3198-3204</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Folic acid was covalently conjugated to 66-nm liposomes via spacers of various lengths in an attempt to target the liposomes
to KB cells expressing folate receptors. Spacers of short and intermediate lengths were unable to mediate association of folate-conjugated
liposomes with receptor-bearing cells, however, use of a 250 A polyethyleneglycol spacer (PEG, M(r) approximately 3350) permitted
avid uptake of the liposomes at approximately 2.5 x 10(5) sites/cell. The binding of folate-PEG liposomes to KB cells could
be competitively inhibited by excess free folate or by antiserum against the folate receptor, demonstrating the interaction
is mediated by the cell surface folate-binding protein. Following binding, cell-associated folate-PEG liposomes were internalized
by folate-receptor-mediated endocytosis at 37 degrees C but not at 4 degrees C. These folate-PEG liposomes show potential
for delivering large quantities of low molecular weight compounds nondestructively into folate receptor-bearing cells.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8106354</pmid><doi>10.1016/S0021-9258(17)41848-5</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1994-02, Vol.269 (5), p.3198-3204 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76358288 |
| source | ScienceDirect (Online service) |
| subjects | Biological and medical sciences Biological Transport - drug effects Carrier Proteins - metabolism Diverse techniques Drug Carriers Endocytosis - drug effects Folate Receptors, GPI-Anchored Folic Acid - analogs & derivatives Folic Acid - pharmacology Fundamental and applied biological sciences. Psychology Humans Immune Sera - pharmacology KB Cells Kinetics Liposomes Molecular and cellular biology Polyethylene Glycols Receptors, Cell Surface |
| title | Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T01%3A30%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Delivery%20of%20liposomes%20into%20cultured%20KB%20cells%20via%20folate%20receptor-mediated%20endocytosis&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=LEE,%20R.%20J&rft.date=1994-02-04&rft.volume=269&rft.issue=5&rft.spage=3198&rft.epage=3204&rft.pages=3198-3204&rft.issn=0021-9258&rft.eissn=1083-351X&rft.coden=JBCHA3&rft_id=info:doi/10.1016/S0021-9258(17)41848-5&rft_dat=%3Cproquest_cross%3E16818699%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c438t-ac7f45cc390f97bc0effee13227bbefb703661a08ae0ed26f2e4e8ba664a89fd3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16818699&rft_id=info:pmid/8106354&rfr_iscdi=true |