Factors Affecting the Photokilling of Cultured Chinese Hamster Cells by Phthalocyanines

Chloroaluminum phthalocyanine (CAPC) was recently shown to sensitize the inactivation of cultured Chinese hamster cells by visible light. Several factors affecting the photodynamic action of CAPC have been defined in the present study. Thus the photosensitized inactivation of Chinese hamster cells i...

Full description

Saved in:
Bibliographic Details
Published in:Radiation research 1985-09, Vol.103 (3), p.403-409
Main Authors: Ben-Hur, E., Rosenthal, I.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological effects of radiation
Bromodeoxyuridine - pharmacology
Cell culture techniques
Cell growth
Cell Line
Cell Survival - drug effects
Cell Survival - radiation effects
Cricetinae
Cricetulus
Cultured cells
DNA damage
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
Indoles - pharmacology
Light
Non ionizing radiations. Hertzian waves. Biooptics
Photochemotherapy
Photosensitivity disorders
Radiation damage
Sodium Salicylate - pharmacology
Space life sciences
Superoxide Dismutase - pharmacology
Superoxides
Tissues, organs and organisms biophysics
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chloroaluminum phthalocyanine (CAPC) was recently shown to sensitize the inactivation of cultured Chinese hamster cells by visible light. Several factors affecting the photodynamic action of CAPC have been defined in the present study. Thus the photosensitized inactivation of Chinese hamster cells is not affected by superoxide dismutase, suggesting that O2- radicals are not involved in the process. Postillumination treatments with D2 O or heat (42°C, 90 min) enhanced CAPC-induced photosensitivity, indicating the existence of a repair mechanism for photodamage. Preillumination treatments with sodium salicylate and 5-bromodeoxyuridine also enhanced photosensitivity. The later observation suggests that CAPC-induced DNA damage is potentially lethal. However, 3-aminobenzamide, a potent inhibitor of poly(ADP-ribose) synthesis which is involved in repair of DNA strand breakage, had no effect on the photosensitivity. Photosensitized inactivation by CAPC is dependent on the pH value of the medium during irradiation. Thus, in the range of pH values 6-8, the sensitivity was increased at the lower values.
ISSN:0033-7587
1938-5404
DOI:10.2307/3576762