Sites of phospholipid biosynthesis during induction of intracytoplasmic membrane formation in Rhodopseudomonas sphaeroides

A rapid, gratuitous and cell-division uncoupled induction of intracytoplasmic photosynthetic membrane formation was demonstrated in low-aeration suspensions of chemotrophically grown Rhodopseudomonas sphaeroides. Despite a nearly 2-fold increase in phospholipid levels, no significant increases were...

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Bibliographic Details
Published in:Archives of microbiology 1985-07, Vol.142 (2), p.136-140
Main Authors: RADCLIFFE, C. W, BROGLIE, R. M, NIEDERMAN, R. A
Format: Article
Language:English
Subjects:
Bacteriology
Biogenesis of cell structures, supramolecular organization
Biological and medical sciences
CDPdiacylglycerol-Serine O-Phosphatidyltransferase - metabolism
Fundamental and applied biological sciences. Psychology
Intracellular Membranes - metabolism
Membrane Lipids - biosynthesis
Microbiology
Phosphatidylglycerols - biosynthesis
Phosphatidylserines - biosynthesis
Phospholipids - biosynthesis
Phospholipids - isolation & purification
Phosphotransferases - metabolism
Rhodobacter sphaeroides - enzymology
Rhodobacter sphaeroides - metabolism
Transferases (Other Substituted Phosphate Groups)
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Summary:A rapid, gratuitous and cell-division uncoupled induction of intracytoplasmic photosynthetic membrane formation was demonstrated in low-aeration suspensions of chemotrophically grown Rhodopseudomonas sphaeroides. Despite a nearly 2-fold increase in phospholipid levels, no significant increases were detected in the specific activities of CDP-1,2-diacyl-sn-glycerol:sn-glycerol-3-phosphate phosphatidyltransferase (phosphatidylglycerophosphate synthase, EC 2.7.8.5) and CDP-1,2-diacyl-sn-glycerol:L-serine O-phosphatidyltransferase (phosphatidylserine synthase, EC 2.7.8.8), the first committed enzymes of anionic and zwitterionic phospholipid biosyntheses, respectively. The distribution of phosphatidylglycerophosphate and phosphatidylserine synthase activities after rate-zone sedimentation of cell-free extracts indicated that intracytoplasmic membrane phospholipids were synthesized mainly within distinct domains of the conserved cytoplasmic membrane. Labeling studies with 32Pi and L-[3H]phenylalanine suggested that preexisting phospholipid was utilized initially as the matrix for insertion of intracytoplasmic membrane protein that was synthesized and assembled de novo during induction.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00447056