Alleged reaction in gel-immunodiffusion of an IgM monoclonal antibody with alfalfa mosaic virus and cucumber mosaic virus is an artefact

Department of Crop Protection, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia 5064, Australia A previously reported spurious serological cross-reaction between alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV), which had been defined by the react...

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Bibliographic Details
Published in:Journal of general virology 1994-02, Vol.75 (2), p.463-465
Main Authors: Hajimorad, M. R, Dietzgen, R. G, Wahyuni, W. S, Francki, R. I. B
Format: Article
Language:English
Subjects:
Alfalfa mosaic virus - immunology
Antibodies, Monoclonal - immunology
Biological and medical sciences
Cross Reactions
Cucumovirus - immunology
Fundamental and applied biological sciences. Psychology
Immunodiffusion
Immunoglobulin M - immunology
Microbiology
Techniques used in virology
Virology
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Summary:Department of Crop Protection, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia 5064, Australia A previously reported spurious serological cross-reaction between alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV), which had been defined by the reaction in gel-immunodiffusion tests of a single IgM monoclonal antibody (MAb), MAb 8, was no longer detected in the presence of 0.1 M -NaCl. The non-specific reactivity of this IgM was also confirmed in Western blotting assays. When skimmed milk was used as a blocking agent and as a diluent of antibodies, MAb 8 failed to recognize AMV and CMV coat proteins. Hence, it is concluded that the alleged cross-reaction between AMV and CMV is due to non-specific binding of MAb 8 and that there is no evidence for a serological relationship between these two viruses. Present address: Agricultural Research Organization, Plant Pests and Diseases Research Institute, P.O. Box 1454, Tehran 19395, Iran. Present address: Queensland Department of Primary Industries, Plant Protection Division and Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, University of Queensland, Queensland 4072, Australia. Present address: Faculty of Agriculture, University of Jember, 3/23 Kalimantan Street, Jember 68121, East Java, Indonesia. || Deceased, 14 November 1990. Received 20 July 1993; accepted 21 September 1993.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-75-2-463