Heparin Cofactor II Activity in Patients With Disseminated Intravascular Coagulation and Hepatic Failure

Heparin cofactor II (HCII) is a glycoprotein in human plasma which inactivates thrombin rapidly in the presence of heparin or dermatan sulfate. We have developed a functional assay for HCII in which inhibition of thrombin by plasma is determined in the presence of dermatan sulfate. The assay is spec...

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Bibliographic Details
Published in:Blood 1985-10, Vol.66 (4), p.769-774
Main Authors: Tollefsen, Douglas M., Pestka, Cynthia A.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antithrombin III - metabolism
Biological and medical sciences
Dermatan Sulfate - pharmacology
Disseminated Intravascular Coagulation - drug therapy
Disseminated Intravascular Coagulation - metabolism
Emergency and intensive care: hematologic diseases, coagulopathies
Female
Heparin - therapeutic use
Humans
Intensive care medicine
Liver Diseases - metabolism
Male
Medical sciences
Middle Aged
Thrombin - antagonists & inhibitors
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Summary:Heparin cofactor II (HCII) is a glycoprotein in human plasma which inactivates thrombin rapidly in the presence of heparin or dermatan sulfate. We have developed a functional assay for HCII in which inhibition of thrombin by plasma is determined in the presence of dermatan sulfate. The assay is specific for HCII by the following criteria: (a) under the conditions of the assay, 126l-thrombin forms complexes in plasma which comigrate with the thrombin-HCII complex during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); (b) activity detected by the assay is decreased in plasma absorbed with monospe-cific antibodies against HCII; and (c) purified antithrombin III (ATIII) is unreactive in the assay system. Addition of Polybrene to the assay permits determination of HCII activity in samples containing
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V66.4.769.769