The complete amino acid sequence of pituitary cystatin from chum salmon

Cystatin, a cysteine proteinase inhibitor, was isolated from chum salmon (Oncorhynchus keta) pituitary glands by ion-exchange chromatography on Mono-Q, gel filtration on Superdex 75, and reverse-phase HPLC on an ODS following ethanol-ammonium acetate extraction. Salmon pituitary cystatin was equipot...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1994-01, Vol.58 (1), p.164-169
Main Authors: Koide, Y. (Kitasato Univ., Sanriku, Iwate (Japan). School of Fisheries), Noso, T
Format: Article
Language:English
Subjects:
ACIDE AMINE
Amino Acid Sequence
AMINOACIDOS
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Biotechnology
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
CISTEINA
Cystatins - genetics
Cystatins - isolation & purification
Cystatins - metabolism
CYSTEINE
Electrophoresis, Polyacrylamide Gel
Enzymes and enzyme inhibitors
ESTRUCTURA QUIMICA
Fundamental and applied biological sciences. Psychology
GLANDULA PITUITARIA
Humans
Hydrolases
HYPOPHYSE
INHIBIDORES DE ENZIMAS
INHIBITEUR D'ENZYME
Marine
Molecular Sequence Data
Oncorhynchus keta
Papain - antagonists & inhibitors
Pituitary Gland - chemistry
Salmon
SALMON DEL PACIFICO
SAUMON DU PACIFIQUE
Sequence Homology, Amino Acid
STRUCTURE CHIMIQUE
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Description
Summary:Cystatin, a cysteine proteinase inhibitor, was isolated from chum salmon (Oncorhynchus keta) pituitary glands by ion-exchange chromatography on Mono-Q, gel filtration on Superdex 75, and reverse-phase HPLC on an ODS following ethanol-ammonium acetate extraction. Salmon pituitary cystatin was equipotent to chicken egg-white cystatin in the papain inhibitory assay. The cystatin consists of 111 amino acid residues with two disulfide linkages formed between 66-75 and 89-109, and has 43% identical sequences with chicken egg-white cystatin with consensus sequences of reactive sites, Gly(4), Gln-X-Val-X-Gly (48-52), and Ile(Val)-Pro-Trp (96-98)
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.58.164