Histone H1 is located in the interior of the chromatin 30-nm filament

THE linker histone H1 binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of chromatin 1 . It has been implicated in the repression of transcription 2–5 , and phosphorylation of H1 may be involved in cell-cycle-dependent chromatin condensation and dec...

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Bibliographic Details
Published in:Nature (London) 1994-03, Vol.368 (6469), p.351-354
Main Authors: Graziano, V, Gerchman, S. E, Schneider, D. K, Ramakrishnan, V
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Chromatin - chemistry
Chromatin. Chromosome
Deuterium
Escherichia coli
Fundamental and applied biological sciences. Psychology
Histones - analysis
Humanities and Social Sciences
letter
Molecular and cellular biology
Molecular genetics
multidisciplinary
Neutrons
Proteins
Recombinant Proteins - genetics
Science
Science (multidisciplinary)
Water
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Summary:THE linker histone H1 binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of chromatin 1 . It has been implicated in the repression of transcription 2–5 , and phosphorylation of H1 may be involved in cell-cycle-dependent chromatin condensation and decondensation 6 . A long-standing issue concerns the location of H1 in the chromatin filament 7 . The original solenoidal model 8 proposes that H1 is inside the 30-nm filament, but other models, also helical, suggest a variable 9 or more accessible 10 location for H1. Investigations to determine the location of the linker histone based on its access-ibility to antibodies 11–15 or immobilized proteases 16 under various ionic conditions have yielded conflicting results. Here we use neutron scattering in a direct structural determination to show that H1 is located in the interior of the filament.
ISSN:0028-0836
1476-4687
DOI:10.1038/368351a0