Block in nuclear localization of period protein by a second clock mutation, timeless

In wild-type Drosophila, the period protein (PER) is found in nuclei of the eyes and brain, and PER immunoreactivity oscillates with a circadian rhythm. The studies described here indicate that the nuclear localization of PER is blocked by timeless (tim), a second chromosome mutation that, like per...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1994-03, Vol.263 (5153), p.1606-1609
Main Authors: Vosshall, L.B, Price, J.L, Sehgal, A, Saez, L, Young, M.W
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antibodies
Biological and medical sciences
Biological Clocks - genetics
Biological rhythms
Biorhythms
Cell nucleus
Cell Nucleus - metabolism
chromosomes
Circadian rhythm
Circadian Rhythm - genetics
Circadian rhythms
Classical genetics, quantitative genetics, hybrids
Cytoplasm - metabolism
Drosophila
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins
Fundamental and applied biological sciences. Psychology
Gene Expression
genes
Genes, Insect
Genetic aspects
Genetic mutation
Genetics of eukaryotes. Biological and molecular evolution
Invertebrata
Laboratory staining techniques
Mutation
Neurons
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Period Circadian Proteins
Phenotype
Photoreceptors
Physiological aspects
Recombinant Fusion Proteins - metabolism
RNA
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Description
Summary:In wild-type Drosophila, the period protein (PER) is found in nuclei of the eyes and brain, and PER immunoreactivity oscillates with a circadian rhythm. The studies described here indicate that the nuclear localization of PER is blocked by timeless (tim), a second chromosome mutation that, like per null mutations, abolishes circadian rhythms. PER fusion proteins without a conserved domain (PAS) and some flanking sequences are nuclear in tim mutants. This suggests that a segment of PER inhibits nuclear localization in tim mutants. The tim gene may have a role in establishing rhythms of PER abundance and nuclear localization in wild-type flies.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8128247