Evidence for phage-mediated gene transfer among Pseudomonas aeruginosa strains on the phylloplane

As the use of genetically engineered microorganisms for agricultural tasks becomes more frequent, the ability of bacteria to exchange genetic material in the agricultural setting must be assessed. Transduction (bacterial virus-mediated horizontal gene transfer) is a potentially important mechanism o...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1994-02, Vol.60 (2), p.496-500
Main Authors: Kidambi, S.P, Ripp, S, Miller, R.V
Format: Article
Language:English
Subjects:
Agricultural production
Bacteria
BACTERIOFAGOS
BACTERIOPHAGE
Bacteriophages - genetics
Biological and medical sciences
Biotechnology
Cellular biology
Environment and pollution
ESPECE
ESPECIES
FEUILLE
Fundamental and applied biological sciences. Psychology
GENETICA
Genetically engineered organisms behavior (microorganisms, plants, animals)
Genetics
GENETIQUE
GLYCINE MAX
HOJAS
IMPACT SUR L'ENVIRONNEMENT
IMPACTO AMBIENTAL
Industrial applications and implications. Economical aspects
Models, Biological
phage F116
Plants - microbiology
PSEUDOMONAS AERUGINOSA
Pseudomonas aeruginosa - genetics
Transfection - genetics
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
VARIEDADES NATURALIZADAS
VARIETE INTRODUITE
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Summary:As the use of genetically engineered microorganisms for agricultural tasks becomes more frequent, the ability of bacteria to exchange genetic material in the agricultural setting must be assessed. Transduction (bacterial virus-mediated horizontal gene transfer) is a potentially important mechanism of gene transfer in natural environments. This study investigated the potential of plant leaves to act as surfaces on which transduction can take place among microorganisms. Pseudomonas aeruginosa and its generalized transducing bacteriophage F116 were used as a model system. The application of P. aeruginosa lysogens of F116 to plant leaves resulted in genetic exchange among donor and recipient organisms resident on the same plant. Transduction was also observed when these bacterial strains were inoculated onto adjacent plants and contact was made possible through high-density planting
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.60.2.496-500.1994