Membrane filtration method for bacteriological testing of water: enhanced colony visualization and stability on purification of phenol red indicator

Commercially‐available phenol red indicator, purified by adsorption chromatography was incorporated into lauryl sulphate broth (LSB) used in the membrane filtration method for the detection of Escherichia coli and other coliform bacteria. Relative to LSB containing the impure dye or its major contam...

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Bibliographic Details
Published in:Letters in applied microbiology 1994-02, Vol.18 (2), p.117-119
Main Authors: Hay, J., Khan, W., Mead, A.J.C., Seal, D.V., Sugden, J.K.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Biotechnology
Escherichia coli
Fundamental and applied biological sciences. Psychology
Microbiology
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Summary:Commercially‐available phenol red indicator, purified by adsorption chromatography was incorporated into lauryl sulphate broth (LSB) used in the membrane filtration method for the detection of Escherichia coli and other coliform bacteria. Relative to LSB containing the impure dye or its major contaminant, the purified phenol red provided clear visualization of discrete yellow colonies observed against a white background. The colonies remained stable for at least 24 h at 25°C under standard laboratory lighting conditions. This simple procedure will enhance the detection of coliforms in samples.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1994.tb00821.x