Selection of unique antigenic variants of Newcastle disease virus with neutralizing monoclonal antibodies and anti-immunoglobulin

Monoclonal antibodies were used to isolate nonneutralizable antigenic variants in the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. It had been found that a large percentage of virus retains infectivity despite binding neutralizing antibody. This high persistent fraction of no...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-10, Vol.82 (20), p.7106-7110
Main Authors: Iorio, R.M, Bratt, M.A
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal
Antigen-Antibody Complex
Antigens, Viral - genetics
Ascites
Biological and medical sciences
Chick Embryo
Epitopes
Epitopes - analysis
Fundamental and applied biological sciences. Psychology
Genetic Variation
Glycoproteins
IMMUNOGLOBULINE
IMMUNOGLOBULINS
INMUNOGLOBULINA
Microbiology
MONOCLONAL ANTIBODIES
Neutralizing antibodies
NEWCASTLE DISEASE VIRUS
Newcastle disease virus - genetics
Newcastle disease virus - immunology
Techniques used in virology
Virions
Virology
VIRUS DE LA ENFERMEDAD DE NEWCASTLE
VIRUS MALADIE DE NEWCASTLE
Viruses
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Description
Summary:Monoclonal antibodies were used to isolate nonneutralizable antigenic variants in the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. It had been found that a large percentage of virus retains infectivity despite binding neutralizing antibody. This high persistent fraction of nonneutralized virus precluded the isolation of variants by the standard treatment with antibody alone. Rabbit anti-mouse immunoglobulin was used to reduce the percentage of virus that remains infectious despite the presence of bound antibody. This procedure made possible the isolation of variants of two distinct types: classical variants, not neutralized because they do not bind the antibody used to select them; and unique variants that, although still capable of binding the selecting antibody, are only slightly neutralized. The general applicability of this method for the isolation of antigenic variants in nonneutralizing epitopes is also discussed.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.20.7106