Detection of Salmonella enteritidis in feces from poultry using booster polymerase chain reaction and oligonucleotide primers specific for all members of the genus Salmonella

• Published in: Poultry science 1994-02, Vol.73 (2), p.354-357
• Main Authors: Cohen, N.D, McGruder, E.D, Neibergs, H.L, Behle, R.W, Wallis, D.E, Hargis, B.M
• Format: Article
• Language: English
• Subjects: AMPLIFICATION CHAINE POLYMERASE; Animals; Base Sequence; Chickens - microbiology; DNA Primers; FECES; Feces - microbiology; Female; FEMELLE; HECES; HEMBRA; METHODE D'ESSAI; METODOS DE ENSAYO; Molecular Sequence Data; POLLO; Polymerase Chain Reaction - veterinary; POULET; REACCION DE CADENAS DE POLIMERASA; SALMONELLA ENTERITIDIS; Salmonella enteritidis - isolation & purification; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Species Specificity
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.0730354

Salmonella enteritidis was identified in feces from hens using the polymerase chain reaction (PCR) and oligonucleotide primers specific for all members of the genus Salmonella. Feces from specific-pathogen-free Leghorn hens were determined to be negative for Salmonella by microbiological culture and by the PCR. Fecal samples were inoculated with known numbers of colony-forming units of S. enteritidis. The DNA was extracted from fecal samples and amplified by the PCR using genus-specific primers. Salmonella were detected in all samples known to be positive; the sensitivity of the assay extended to 1 cfu of S. enteritidis/g feces. Feces that were not inoculated with Salmonella were negative. Microbiological culture was less sensitive than the PCR assay; results of culture of feces with less than 10(2) cfu/g were negative. Although S. enteritidis was used in this study, the oligonucleotide primers used in this study have been previously demonstrated to be genus-specific for Salmonella