Lactosylceramide is synthesized in the lumen of the Golgi apparatus

Recently, synthesis of lactosylceramide has been described to occur on the cytosolic face of the Golgi [(1991) J. Biol. Chem. 266, 20907-20912]. The reactions following in the biosynthesis of higher glycosphingolipids are known to take place in the lumen of the Golgi. For our understanding of the fu...

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Bibliographic Details
Published in:FEBS letters 1994-03, Vol.342 (1), p.91-96
Main Authors: Lannert, Heinrich, Bünning, Garsten, Jeckel, Dieter, Wieland, Felix T.
Format: Article
Language:English
Subjects:
(truncated) C8,C8-ceramide
(truncated) C8,C8-glucosylceramide
(truncated) C8,C8-lac-tosylceramide
(truncated) C8,C8-sphingomyelin
Analytical, structural and metabolic biochemistry
Animals
Antigens, CD
Biological and medical sciences
Chinese hamster ovary cells
CHO cell
CHO Cells
Cricetinae
Fundamental and applied biological sciences. Psychology
Galactosyltransferases - metabolism
GlcCer
glucosylceramide
Glucosylceramides - metabolism
Glucosyltransferases - metabolism
Glycosphingolipid
Glycosphingolipids - biosynthesis
Golgi
Golgi Apparatus - metabolism
LacCer
lactosylceramide
Lactosylceramide synthase
Lactosylceramides
Lipids
Madin Darby canine kidney cells
Male
MDCK-cells
Other biological molecules
Rats
Rats, Wistar
Semi-intact cell
semi-intact cells
SIC
Sph
Sphingolipids
sphingomyelin
t-Cer
t-GlcCer
t-LacCer
t-Sph
Temperature
Transferases (Other Substituted Phosphate Groups) - metabolism
Uridine Diphosphate Galactose - metabolism
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Summary:Recently, synthesis of lactosylceramide has been described to occur on the cytosolic face of the Golgi [(1991) J. Biol. Chem. 266, 20907-20912]. The reactions following in the biosynthesis of higher glycosphingolipids are known to take place in the lumen of the Golgi. For our understanding of the functional organization of the multiglycosyltransferase system of glycosphingolipid synthesis in the Golgi, the knowledge of the topology of individual reactions is a prerequisite. We have developed a simple and quick assay system for sphingolipid biosynthesis and have obtained evidence that lactosylceramide is synthesized in the lumen of the Golgi. Because lactosylceramide is generated by galactosylation of glucosylceramide which, in turn, is synthesized from ceramide and UDP-Glc on the cytosolic surface of the Golgi apparatus, further efforts will be directed to the characterization of a glucosylceramide-translocator in the Golgi membranes rather than a lactosylceramide-translocator.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)80591-1