The influence of the genes An1, An2, and An4 on the activity of the enzyme UDP-glucose: flavonoid 3-O-glucosyltransferase in flowers of Petunia hybrida

A relation between gene dosage and UDP-glucose:flavonoid 3-O-glucosyl-transferase (UFGT) activity was found in homozygous dominant and recessive parental lines and their F1 progeny for both of the genes An1 and An2. In both F2 crosses, progeny plants could be classified as belonging to groups showin...

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Bibliographic Details
Published in:Biochemical genetics 1985-08, Vol.23 (7/8), p.591-598
Main Authors: Gerats, A.G.M, Vrijlandt, E, Wallroth, M, Schram, A.W
Format: Article
Language:English
Subjects:
Alleles
Anthocyanins - metabolism
FLAVONOIDE
FLAVONOIDES
FLAVONOIDS
GENE
Gene Expression Regulation
GENES
GLUCOSA
GLUCOSE
Glucosyltransferases - genetics
Hot Temperature
PETUNIA
PETUNIA HYBRIDA
Plants - genetics
Species Specificity
TRANSFERASAS
TRANSFERASE
TRANSFERASES
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Summary:A relation between gene dosage and UDP-glucose:flavonoid 3-O-glucosyl-transferase (UFGT) activity was found in homozygous dominant and recessive parental lines and their F1 progeny for both of the genes An1 and An2. In both F2 crosses, progeny plants could be classified as belonging to groups showing either a low or a medium to high UFGT activity. Test crosses showed that heterozygous and homozygous dominant plants were present throughout the medium- to high-active group. The dosage relation in F2 plants is most probably confounded by the segregation of modifiers. Thermal inactivation experiments indicated that structurally different UFGT enzymes are formed in homozygous dominant lines as well as in lines homozygous recessive for either An1 or An2. Lines homozygous recessive for the gene An4 contain a UFGT with a half-life time at 55 degrees C of less than 8 min, whereas UFGTs from lines homozygous dominant for An4 show a half-life time of 25 min or above, with one exception. This relation was confirmed in the F2 progeny; heterozygotes for An4 showed an intermediate half-life time. It is concluded that An4 might be the structural gene for the enzyme; An1 and An2 are both regulatory genes. UFGT activity in flowerbuds of An4/An4 plants seems to be lower than in an4/an4 plants. Anthers of flowers of an4/an4 lines, however, are virtually devoid of UFGT activity.
ISSN:0006-2928
1573-4927
DOI:10.1007/bf00504293