Establishment of Two Permanent Human Bone Marrow Stromal Cell Lines With Long-term Post Irradiation Feeder Capacity

• Published in: Blood 1994-04, Vol.83 (7), p.1799-1807
• Main Authors: Thalmeier, K., Meiβner, P., Reisbach, G., Falk, M., Brechtel, A., Dörmer, P.
• Format: Article
• Language: English
• Subjects: Animal cells; Biological and medical sciences; Bone Marrow Cells; Cell cultures. Hybridization. Fusion; Cell Line; Fetal Blood - cytology; Fundamental and applied biological sciences. Psychology; Hematopoiesis; Humans; Male; Molecular and cellular biology; Radiation Tolerance; Stromal Cells - physiology; Stromal Cells - radiation effects
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V83.7.1799.1799

We describe the establishment of two permanent Simian virus 40-transformed human stromal cell lines, designated L87/4 and L88/5, derived from the bone marrow of a hemato-logically normal male patient. Both cell lines show a fibro-blastoid morphology and do not express hematopoietic cell markers. L87/4 but not L88/5 expresses the macrophage marker CD68. The most remarkable feature of these new stromal cell lines is their ability to persist as growth-arrested adherent feeder cells after ionizing-irradiation at doses up to, and exceeding 20 Gy (L87/4). This renders them particularly useful for studying aspects of feeder dependence of hematopoietic cell development in long-term culture. Both cell lines are able to function as feeder cells, supporting the long-term proliferation of CD34+ human cord blood cells as well as the clonogenic growth of the human Burkitt lymphoma B-cell line BL70.