Exposure to Fatty Acid Increases Human Low Density Lipoprotein Transfer across Cultured Endothelial Monolayers

Human low density lipoproteins radiolabeled with I transfer across confluent monolayers of cultured porcine pulmonary artery endothelial cells. The amount transferred was dependent on the low density lipoprotein concentration and was not saturable at concentrations up to 300 μg protein per 0.5 ml me...

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Bibliographic Details
Published in:Circulation research 1985-11, Vol.57 (5), p.776-780
Main Authors: Hennig, Bernhard, Shasby, Michael D, Spector, Arthur A
Format: Article
Language:English
Subjects:
Animals
Arteries - metabolism
Arteriosclerosis - blood
Arteriosclerosis - etiology
Biological and medical sciences
Blood vessels and receptors
Cells, Cultured
Endothelium - metabolism
Fatty Acids - blood
Fundamental and applied biological sciences. Psychology
Humans
Linoleic Acid
Linoleic Acids - blood
Lipoproteins, LDL - blood
Oleic Acid
Oleic Acids - blood
Pulmonary Artery - metabolism
Swine
Vertebrates: cardiovascular system
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Summary:Human low density lipoproteins radiolabeled with I transfer across confluent monolayers of cultured porcine pulmonary artery endothelial cells. The amount transferred was dependent on the low density lipoprotein concentration and was not saturable at concentrations up to 300 μg protein per 0.5 ml medium. Gel filtration showed that more than 90% of the I which crossed the endothelial monolayer remained associated with low density lipoproteins, indicating that appreciable amounts of lipoprotein were not degraded during the transfer process. When the endothelial monolayer was exposed for 24 hours to culture media supplemented with 100-300 μm fatty acid complexed with 100 μm albumin, the amount of low density lipoprotein subsequently transferred increased by 65% to 150%. The extent of the increase was dependent on the type of fatty acid added and its concentration. At 200 μm, albumin-bound oleic and linoleic acids increased low density lipoprotein transfer, whereas palmitic, linolenic, arachidonic, and eicosapentaenoic acids did not. These results are consistent with the hypothesis that exposure of the endothelium to elevated concentrations of fatty acid may allow excessive amounts of cholesterol-rich lipoproteins to enter the arterial intima. (Circ Res 57776-780, 1985)
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.57.5.776