Isolation and characterization of a novel nucleoside, 7-β-D-ribofuranosylhypoxanthine, from the urine of a chronic myelogenous leukemia patient

A novel nucleoside, in the amount of 400 micrograms, was isolated from a 24-h collection of urine of a chronic myelogenous leukemia patient. On the basis of ultraviolet, nuclear magnetic resonance, and mass spectrometry and chromatography, its structure was established to be 7-beta-D-ribofuranosylhy...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1985-11, Vol.45 (11), p.5958-5963
Main Authors: CHHEDA, G. B, DUTTA, S. P, MITTELMAN, A, MONTGOMERY, J. A, SETHI, S. K, MCCLOSKEY, J. A, PATRZYC, H. B
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Hematologic and hematopoietic diseases
Humans
Inosine - urine
Leukemia, Myeloid - urine
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Magnetic Resonance Spectroscopy
Male
Mass Spectrometry
Medical sciences
Spectrophotometry, Ultraviolet
Vitamin B 12 - blood
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Summary:A novel nucleoside, in the amount of 400 micrograms, was isolated from a 24-h collection of urine of a chronic myelogenous leukemia patient. On the basis of ultraviolet, nuclear magnetic resonance, and mass spectrometry and chromatography, its structure was established to be 7-beta-D-ribofuranosylhypoxanthine. The ultraviolet and mass spectral data and the thin layer chromatographic mobilities of the natural material were identical to those of a synthetic sample. High performance liquid chromatographic retention times and the coinjection high performance liquid chromatography of the natural material with the synthetic samples of the alpha and beta-anomers of 7-ribofuranosylhypoxanthines further confirmed the identity of the isolated material as 7-beta-D-ribofuranosylhypoxanthine.
ISSN:0008-5472
1538-7445