rse, a novel receptor-type tyrosine kinase with homology to Axl/Ufo, is expressed at high levels in the brain

• Published in: The Journal of biological chemistry 1994-04, Vol.269 (14), p.10720-10728
• Main Authors: MARK, M. R, SCADDEN, D. T, ZHENGYU WANG, QIMIN GU, GODDARD, A, GODOWSKI, P. J
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; Brain - enzymology; cDNA; chromosome 15; DNA, Complementary; Enzymes and enzyme inhibitors; Epitopes; Fundamental and applied biological sciences. Psychology; genes; Humans; man; Mice; Molecular Sequence Data; nucleotide sequence; Oncogene Proteins - chemistry; Oncogene Proteins - genetics; prediction; protein-tyrosine kinase; Proto-Oncogene Proteins; Receptor Protein-Tyrosine Kinases - biosynthesis; Receptor Protein-Tyrosine Kinases - chemistry; Receptor Protein-Tyrosine Kinases - genetics; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - genetics; rse gene; Sequence Homology, Amino Acid; Transferases
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(17)34118-2

We have isolated cDNA clones that encode the human and murine forms of a novel receptor-type tyrosine kinase termed Rse. Sequence analysis indicates that human Rse contains 890 amino acids, with an extracellular region composed of two immunoglobulin-like domains followed by two fibronectin type III domains. Murine Rse contains 880 amino acids and shares 90% amino acid identity with its human counterpart. Rse is structurally similar to the receptor-type tyrosine kinase Axl/Ufo, and the two proteins have 35 and 63% sequence identity in their extracellular and intracellular domains, respectively. To study the synthesis and activation of this putative receptor-type tyrosine kinase, we constructed a version of Rse (termed gD-Rse, where gD represents glycoprotein D) that contains an NH2-terminal epitope tag. NIH3T3 cells were engineered to express gD-Rse, which could be detected at the cell surface by fluorescence-activated cell sorting. Moreover, gD-Rse was rapidly phosphorylated on tyrosine residues upon incubation of the cells with an antibody directed against the epitope tag, suggesting that rse encodes an active tyrosine kinase. In the human tissues we examined, the highest level of expression of rse mRNA was observed in the brain; rse mRNA was also detected in the premegakaryocytopoietic cell lines CMK11-5 and Dami. The gene for rse was localized to human chromosome 15.