Biogenesis of liver δ-aminolevulinate synthase: the role of cAMP in the induction

In primary cultures of chick embryo hepatocytes pulse labeled with [35S]methionine, immunochemical analyses indicated that adenosine 3':5'-cyclic monophosphate (cAMP) did not affect either the rate of production or the maturation of delta-aminolevulinate synthase (ALA synthase). In additio...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1985-10, Vol.191 (1), p.117-120
Main Authors: FRIEDLAND, D. M, ADES, I. Z
Format: Article
Language:English
Subjects:
5-Aminolevulinate Synthetase - biosynthesis
Animals
Biological and medical sciences
Cells, Cultured
Chick Embryo
Cyclic AMP - analysis
Cyclic AMP - physiology
Enzyme Induction
Fundamental and applied biological sciences. Psychology
Liver - enzymology
Molecular and cellular biology
Molecular genetics
Translation. Translation factors. Protein processing
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In primary cultures of chick embryo hepatocytes pulse labeled with [35S]methionine, immunochemical analyses indicated that adenosine 3':5'-cyclic monophosphate (cAMP) did not affect either the rate of production or the maturation of delta-aminolevulinate synthase (ALA synthase). In addition, allylisopropylacetamide caused a slight drop in intracellular cAMP while testosterone caused the levels of cAMP to rise to 260% of the basal levels measured in hepatocytes in culture. Thus the results of this study did not indicate a direct short-term role for cAMP in the regulation of production of ALA synthase.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)81005-X