Gas Chromatographic-Mass Spectrometric Determination of 13C-Glucose Level for Evaluating the Effect of α-Glucosidase Inhibitor Acarbose on the Digestion of [U-13C]Starch in Rat

The effect of acarbose on the digestion of starch was examined by a stable isotope tracer technique. [U-13C]-Starch was administered orally to rats with or without acarbose. After the addition of [2H3]-D-glucose as the internal standard, the plasma samples were treated successively for defatting, de...

Full description

Saved in:
Bibliographic Details
Published in:Biological & pharmaceutical bulletin 1994/01/15, Vol.17(1), pp.156-159
Main Authors: GOROMARU, Tsuyoshi, MATSUKI, Kazuhiro, MATSUKI, Yoko
Format: Article
Language:English
Subjects:
Acarbose
Animals
Biological and medical sciences
Borohydrides - chemistry
C starch
C-glucose
Calibration
Carbon Radioisotopes
co-administration
digestion
Digestion - drug effects
Gas Chromatography-Mass Spectrometry
GC-MS
General and cellular metabolism. Vitamins
Glucose - metabolism
Glycoside Hydrolase Inhibitors
Male
Medical sciences
Oxidation-Reduction
Pharmacology. Drug treatments
Rats
Rats, Wistar
Starch - metabolism
Trisaccharides - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of acarbose on the digestion of starch was examined by a stable isotope tracer technique. [U-13C]-Starch was administered orally to rats with or without acarbose. After the addition of [2H3]-D-glucose as the internal standard, the plasma samples were treated successively for defatting, deproteinizing and desalting. Glucose was converted to sorbitol by reduction with sodium borohydride. The cyclic butylboronate of sorbitol was injected into a gas chromatograph-mass spectrometer, and the concentration of labeled glucose was measured by selected monitoring of the quasi-molecular ion. The plasma concentration of labeled glucose was decreased significantly by the addition of acarbose. The effect of acarbose on the digestion of starch was clearly confirmed using [U-13C]starch.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.17.156