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Gas Chromatographic-Mass Spectrometric Determination of 13C-Glucose Level for Evaluating the Effect of α-Glucosidase Inhibitor Acarbose on the Digestion of [U-13C]Starch in Rat

The effect of acarbose on the digestion of starch was examined by a stable isotope tracer technique. [U-13C]-Starch was administered orally to rats with or without acarbose. After the addition of [2H3]-D-glucose as the internal standard, the plasma samples were treated successively for defatting, de...

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Published in:	Biological & pharmaceutical bulletin 1994/01/15, Vol.17(1), pp.156-159
Main Authors:	GOROMARU, Tsuyoshi, MATSUKI, Kazuhiro, MATSUKI, Yoko
Format:	Article
Language:	English
Subjects:	Acarbose Animals Biological and medical sciences Borohydrides - chemistry C starch C-glucose Calibration Carbon Radioisotopes co-administration digestion Digestion - drug effects Gas Chromatography-Mass Spectrometry GC-MS General and cellular metabolism. Vitamins Glucose - metabolism Glycoside Hydrolase Inhibitors Male Medical sciences Oxidation-Reduction Pharmacology. Drug treatments Rats Rats, Wistar Starch - metabolism Trisaccharides - pharmacology
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creator	GOROMARU, Tsuyoshi MATSUKI, Kazuhiro MATSUKI, Yoko
description	The effect of acarbose on the digestion of starch was examined by a stable isotope tracer technique. [U-13C]-Starch was administered orally to rats with or without acarbose. After the addition of [2H3]-D-glucose as the internal standard, the plasma samples were treated successively for defatting, deproteinizing and desalting. Glucose was converted to sorbitol by reduction with sodium borohydride. The cyclic butylboronate of sorbitol was injected into a gas chromatograph-mass spectrometer, and the concentration of labeled glucose was measured by selected monitoring of the quasi-molecular ion. The plasma concentration of labeled glucose was decreased significantly by the addition of acarbose. The effect of acarbose on the digestion of starch was clearly confirmed using [U-13C]starch.
doi_str_mv	10.1248/bpb.17.156
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The effect of acarbose on the digestion of starch was clearly confirmed using [U-13C]starch.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.17.156</identifier><identifier>PMID: 8148808</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Acarbose ; Animals ; Biological and medical sciences ; Borohydrides - chemistry ; C starch ; C-glucose ; Calibration ; Carbon Radioisotopes ; co-administration ; digestion ; Digestion - drug effects ; Gas Chromatography-Mass Spectrometry ; GC-MS ; General and cellular metabolism. Vitamins ; Glucose - metabolism ; Glycoside Hydrolase Inhibitors ; Male ; Medical sciences ; Oxidation-Reduction ; Pharmacology. 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[U-13C]-Starch was administered orally to rats with or without acarbose. After the addition of [2H3]-D-glucose as the internal standard, the plasma samples were treated successively for defatting, deproteinizing and desalting. Glucose was converted to sorbitol by reduction with sodium borohydride. The cyclic butylboronate of sorbitol was injected into a gas chromatograph-mass spectrometer, and the concentration of labeled glucose was measured by selected monitoring of the quasi-molecular ion. The plasma concentration of labeled glucose was decreased significantly by the addition of acarbose. The effect of acarbose on the digestion of starch was clearly confirmed using [U-13C]starch.</description><subject>Acarbose</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Borohydrides - chemistry</subject><subject>C starch</subject><subject>C-glucose</subject><subject>Calibration</subject><subject>Carbon Radioisotopes</subject><subject>co-administration</subject><subject>digestion</subject><subject>Digestion - drug effects</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>GC-MS</subject><subject>General and cellular metabolism. Vitamins</subject><subject>Glucose - metabolism</subject><subject>Glycoside Hydrolase Inhibitors</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Oxidation-Reduction</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Starch - metabolism</subject><subject>Trisaccharides - pharmacology</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNo9kc-L1DAcxYMo67h68S7kIB6EjkmbtMlx6Y7jwojguieR8m2aTLP01ybpgn_W_iP-TaZOnUsCeZ_3XuAh9JaSLU2Z-FRP9ZYWW8rzZ2hDM1YkPKX8OdoQSUWSUy5eolfe3xNCCpJmF-hCUCYEERv0tAePy9aNPYTx6GBqrUq-gvf4dtIqxHcdnFX4WgftejtAsOOAR4NpVib7blaj1_igH3WHzejw7hG6OTLDEYdW450xMWTB_zyttG0gOm6G1tY2RMeVAlcvITF2sVzbo_b_S37eJbHn120Ap1psB_wdwmv0wkDn9Zv1vkR3n3c_yi_J4dv-prw6JIqlNE_ygoHhaa1ACCMJJ4YaIWlRm1xmVAJIYA2njNBcFkYTTRoOtWhSrQwnTGSX6MMpd3Ljwxz_VPXWK911MOhx9lWRs5TnNI3gxxOo3Oi906aanO3B_a4oqZZ9qrhPRYsq7hPhd2vqXPe6OaPrIFF_v-rgFXTGwaCsP2OM5EJkMmLlCbv3AY76rIMLVnV6aaRSZv9a14PnZ1W14Co9ZH8BEN2x6Q</recordid><startdate>199401</startdate><enddate>199401</enddate><creator>GOROMARU, Tsuyoshi</creator><creator>MATSUKI, Kazuhiro</creator><creator>MATSUKI, Yoko</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199401</creationdate><title>Gas Chromatographic-Mass Spectrometric Determination of 13C-Glucose Level for Evaluating the Effect of α-Glucosidase Inhibitor Acarbose on the Digestion of [U-13C]Starch in Rat</title><author>GOROMARU, Tsuyoshi ; MATSUKI, Kazuhiro ; MATSUKI, Yoko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4216-674af52bca88f9050f1f8917bf69319aa9a4d51401697fe0e0d5ab8d2ecf50483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acarbose</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Borohydrides - chemistry</topic><topic>C starch</topic><topic>C-glucose</topic><topic>Calibration</topic><topic>Carbon Radioisotopes</topic><topic>co-administration</topic><topic>digestion</topic><topic>Digestion - drug effects</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>GC-MS</topic><topic>General and cellular metabolism. Vitamins</topic><topic>Glucose - metabolism</topic><topic>Glycoside Hydrolase Inhibitors</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Oxidation-Reduction</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Starch - metabolism</topic><topic>Trisaccharides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GOROMARU, Tsuyoshi</creatorcontrib><creatorcontrib>MATSUKI, Kazuhiro</creatorcontrib><creatorcontrib>MATSUKI, Yoko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GOROMARU, Tsuyoshi</au><au>MATSUKI, Kazuhiro</au><au>MATSUKI, Yoko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gas Chromatographic-Mass Spectrometric Determination of 13C-Glucose Level for Evaluating the Effect of α-Glucosidase Inhibitor Acarbose on the Digestion of [U-13C]Starch in Rat</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1994-01</date><risdate>1994</risdate><volume>17</volume><issue>1</issue><spage>156</spage><epage>159</epage><pages>156-159</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The effect of acarbose on the digestion of starch was examined by a stable isotope tracer technique. 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subjects	Acarbose Animals Biological and medical sciences Borohydrides - chemistry C starch C-glucose Calibration Carbon Radioisotopes co-administration digestion Digestion - drug effects Gas Chromatography-Mass Spectrometry GC-MS General and cellular metabolism. Vitamins Glucose - metabolism Glycoside Hydrolase Inhibitors Male Medical sciences Oxidation-Reduction Pharmacology. Drug treatments Rats Rats, Wistar Starch - metabolism Trisaccharides - pharmacology
title	Gas Chromatographic-Mass Spectrometric Determination of 13C-Glucose Level for Evaluating the Effect of α-Glucosidase Inhibitor Acarbose on the Digestion of [U-13C]Starch in Rat
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