Adhesion Forces Between Individual Ligand-Receptor Pairs

The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to sepa...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1994-04, Vol.264 (5157), p.415-417
Main Authors: Florin, Ernst-Ludwig, Moy, Vincent T., Gaub, Hermann E.
Format: Article
Language:English
Subjects:
Adhesion
Adhesive bonding
Adhesiveness
Adhesives
Atomic force microscopy
Autocorrelation
Avidin - chemistry
Biochemistry
Biological and medical sciences
Biotin - analogs & derivatives
Biotin - chemistry
Cantilevers
Fundamental and applied biological sciences. Psychology
Histograms
Hydrogen bonds
Interactions. Associations
Intermolecular forces
Intermolecular phenomena
Laboratory Equipment
Ligands
Microscopes
Microscopy - methods
Microspheres
Molecular biophysics
Molecular interactions
Molecules
Receptors, Cell Surface - chemistry
Sepharose
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Summary:The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 ± 20 piconewtons for biotin and 85 ± 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8153628