HIV-1 envelope protein gp120 affects phenotype and function of monocytes in vitro

We investigated the effect of human immunodeficiency virus type 1 (HIV‐1) recombinant gp120 (rec.gp120) on phenotype and function of cultured monocytes. Rec.gp120 significantly reduced the accessory function of monocytes to stimulate autologous lymphocytes with anti‐CD3, the Fc receptor–mediated che...

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Bibliographic Details
Published in:Journal of leukocyte biology 1994-04, Vol.55 (4), p.545-551
Main Authors: Dürrbaum-Landmann, I, Kaltenhäuser, E, Flad, H D, Ernst, M
Format: Article
Language:English
Subjects:
AIDS/HIV
antigen presentation
Antigen Presentation - drug effects
anti‐CD3 stimulation
Biological and medical sciences
CD4 and Fc receptors
CD4 Antigens - analysis
HIV Envelope Protein gp120 - pharmacology
HIV Infections - immunology
human immunodeficiency virus 1
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
In Vitro Techniques
Interleukin-6 - biosynthesis
Luminescent Measurements
Lymphocyte Activation - drug effects
Medical sciences
Monocytes - drug effects
Monocytes - immunology
phagocytosis
Phenotype
Receptors, Fc - analysis
Tuberculin - immunology
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Summary:We investigated the effect of human immunodeficiency virus type 1 (HIV‐1) recombinant gp120 (rec.gp120) on phenotype and function of cultured monocytes. Rec.gp120 significantly reduced the accessory function of monocytes to stimulate autologous lymphocytes with anti‐CD3, the Fc receptor–mediated chemiluminescence of monocytes, and the expression of CD4 and Fc receptor I/II, while the expression of the monocyte marker GD14 and major histocompatibility complex class I and II was not influenced. According to these phenotypic results, preincubation of monocytes with rec.gp120 depressed anti‐CD3 antibody‐induced T cell stimulation and Fc receptor‐mediated phagocytosis as determined by chemiluminescence. Interferon‐γ release of lymphocytes induced by purified protein derivative of tuberculin was enhanced by gp120. These effects of isolated gp120 on monocyte immune functions in vitro might contribute to the understanding of the pathophysiology of HIV‐1 infection in viva J. Leukoc. Biol. 55: 545–551; 1994.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.55.4.545