Joint distribution of insertion elements IS4 and IS5 in natural isolates of Escherichia coli

A reference collection of natural isolates of Escherichia coli has been studied in order to determine the distribution, abundance and joint occurrence of DNA insertion elements IS4 and IS5. Among these isolates, 36% were found to contain IS4 and 30% were found to contain IS5. Among strains containin...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 1985-10, Vol.111 (2), p.219-231
Main Authors: DYKHUIZEN, D. E, SAWYER, A. A, GREEN, L, MILLER, R. D, HARTL, D. L
Format: Article
Language:English
Subjects:
Base Composition
Biological and medical sciences
DNA Restriction Enzymes
DNA Transposable Elements
Escherichia coli
Escherichia coli - genetics
Escherichia coli - isolation & purification
Fundamental and applied biological sciences. Psychology
Genic rearrangement. Recombination. Transposable element
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Plasmids
Species Specificity
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 231
container_issue 2
container_start_page 219
container_title Genetics (Austin)
container_volume 111
creator DYKHUIZEN, D. E
SAWYER, A. A
GREEN, L
MILLER, R. D
HARTL, D. L
description A reference collection of natural isolates of Escherichia coli has been studied in order to determine the distribution, abundance and joint occurrence of DNA insertion elements IS4 and IS5. Among these isolates, 36% were found to contain IS4 and 30% were found to contain IS5. Among strains containing IS4 the mean number of copies per strain was 4.4 +/- 0.8; the comparable figure for IS5 was 3.7 +/- 1.0. Although the presence of the elements among the isolates was independent, among those isolates containing both IS4 and IS5, there was a significant negative correlation in the number of copies of the elements. The reference collection was also studied for the presence of the DNA sequences flanking the single copy of IS4 in the chromosome of E. coli K12. Homologous sequences were found in only 26% of the isolates. The sequences flanking the IS4 invariably occur together, and their presence is significantly correlated with the presence of IS4. In eight of the strains that carry these flanking sequences, an IS4 is located between them, and the sequences are present at the homologous position as in the K12 strain. We suggest that IS4 and its flanking sequences share a common mechanism of dissemination, such as plasmids, and we present evidence that they are included in a much larger transposable element.
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_76429964</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76429964</sourcerecordid><originalsourceid>FETCH-LOGICAL-p181t-b0556197cc45ce1d7200d03401fe425e1e216c5d7c89c5a0c475bb50ca73e71d3</originalsourceid><addsrcrecordid>eNqFkE1LxDAQhoMo67r6E4QcxFshab6aoyyrrix4UG9CSdMpG0nTNUkP_nu7Wrx6ehmeZwbmPUFLqjkrSsnoKVoSQmUhFaPn6CKlD0KI1KJaoEWptdRKLtH70-BCxq1LObpmzG4IeOiwCwnizwAeegg54e0Lxya0U4oJ42DyGI3HLg3eZEjHrU2ye4jO7p3BdvDuEp11xie4mnOF3u43r-vHYvf8sF3f7YoDrWguGiKEpFpZy4UF2qqSkJYwTmgHvBRAoaTSilbZSlthiOVKNI0g1igGirZshW5_7x7i8DlCynXvkgXvTYBhTLWS_Pgw_1eknIlSCT2J17M4Nj209SG63sSveu5t4jczN8ka30UTrEt_WsU5qyrKvgHoRXZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14352759</pqid></control><display><type>article</type><title>Joint distribution of insertion elements IS4 and IS5 in natural isolates of Escherichia coli</title><source>Freely Accessible Science Journals</source><source>Alma/SFX Local Collection</source><creator>DYKHUIZEN, D. E ; SAWYER, A. A ; GREEN, L ; MILLER, R. D ; HARTL, D. L</creator><creatorcontrib>DYKHUIZEN, D. E ; SAWYER, A. A ; GREEN, L ; MILLER, R. D ; HARTL, D. L</creatorcontrib><description>A reference collection of natural isolates of Escherichia coli has been studied in order to determine the distribution, abundance and joint occurrence of DNA insertion elements IS4 and IS5. Among these isolates, 36% were found to contain IS4 and 30% were found to contain IS5. Among strains containing IS4 the mean number of copies per strain was 4.4 +/- 0.8; the comparable figure for IS5 was 3.7 +/- 1.0. Although the presence of the elements among the isolates was independent, among those isolates containing both IS4 and IS5, there was a significant negative correlation in the number of copies of the elements. The reference collection was also studied for the presence of the DNA sequences flanking the single copy of IS4 in the chromosome of E. coli K12. Homologous sequences were found in only 26% of the isolates. The sequences flanking the IS4 invariably occur together, and their presence is significantly correlated with the presence of IS4. In eight of the strains that carry these flanking sequences, an IS4 is located between them, and the sequences are present at the homologous position as in the K12 strain. We suggest that IS4 and its flanking sequences share a common mechanism of dissemination, such as plasmids, and we present evidence that they are included in a much larger transposable element.</description><identifier>ISSN: 0016-6731</identifier><identifier>EISSN: 1943-2631</identifier><identifier>PMID: 2996976</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>Bethesda, MD: Genetics Society of America</publisher><subject>Base Composition ; Biological and medical sciences ; DNA Restriction Enzymes ; DNA Transposable Elements ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - isolation &amp; purification ; Fundamental and applied biological sciences. Psychology ; Genic rearrangement. Recombination. Transposable element ; Molecular and cellular biology ; Molecular genetics ; Nucleic Acid Hybridization ; Plasmids ; Species Specificity</subject><ispartof>Genetics (Austin), 1985-10, Vol.111 (2), p.219-231</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8443881$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2996976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DYKHUIZEN, D. E</creatorcontrib><creatorcontrib>SAWYER, A. A</creatorcontrib><creatorcontrib>GREEN, L</creatorcontrib><creatorcontrib>MILLER, R. D</creatorcontrib><creatorcontrib>HARTL, D. L</creatorcontrib><title>Joint distribution of insertion elements IS4 and IS5 in natural isolates of Escherichia coli</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>A reference collection of natural isolates of Escherichia coli has been studied in order to determine the distribution, abundance and joint occurrence of DNA insertion elements IS4 and IS5. Among these isolates, 36% were found to contain IS4 and 30% were found to contain IS5. Among strains containing IS4 the mean number of copies per strain was 4.4 +/- 0.8; the comparable figure for IS5 was 3.7 +/- 1.0. Although the presence of the elements among the isolates was independent, among those isolates containing both IS4 and IS5, there was a significant negative correlation in the number of copies of the elements. The reference collection was also studied for the presence of the DNA sequences flanking the single copy of IS4 in the chromosome of E. coli K12. Homologous sequences were found in only 26% of the isolates. The sequences flanking the IS4 invariably occur together, and their presence is significantly correlated with the presence of IS4. In eight of the strains that carry these flanking sequences, an IS4 is located between them, and the sequences are present at the homologous position as in the K12 strain. We suggest that IS4 and its flanking sequences share a common mechanism of dissemination, such as plasmids, and we present evidence that they are included in a much larger transposable element.</description><subject>Base Composition</subject><subject>Biological and medical sciences</subject><subject>DNA Restriction Enzymes</subject><subject>DNA Transposable Elements</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation &amp; purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nucleic Acid Hybridization</subject><subject>Plasmids</subject><subject>Species Specificity</subject><issn>0016-6731</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAQhoMo67r6E4QcxFshab6aoyyrrix4UG9CSdMpG0nTNUkP_nu7Wrx6ehmeZwbmPUFLqjkrSsnoKVoSQmUhFaPn6CKlD0KI1KJaoEWptdRKLtH70-BCxq1LObpmzG4IeOiwCwnizwAeegg54e0Lxya0U4oJ42DyGI3HLg3eZEjHrU2ye4jO7p3BdvDuEp11xie4mnOF3u43r-vHYvf8sF3f7YoDrWguGiKEpFpZy4UF2qqSkJYwTmgHvBRAoaTSilbZSlthiOVKNI0g1igGirZshW5_7x7i8DlCynXvkgXvTYBhTLWS_Pgw_1eknIlSCT2J17M4Nj209SG63sSveu5t4jczN8ka30UTrEt_WsU5qyrKvgHoRXZw</recordid><startdate>198510</startdate><enddate>198510</enddate><creator>DYKHUIZEN, D. E</creator><creator>SAWYER, A. A</creator><creator>GREEN, L</creator><creator>MILLER, R. D</creator><creator>HARTL, D. L</creator><general>Genetics Society of America</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198510</creationdate><title>Joint distribution of insertion elements IS4 and IS5 in natural isolates of Escherichia coli</title><author>DYKHUIZEN, D. E ; SAWYER, A. A ; GREEN, L ; MILLER, R. D ; HARTL, D. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p181t-b0556197cc45ce1d7200d03401fe425e1e216c5d7c89c5a0c475bb50ca73e71d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Base Composition</topic><topic>Biological and medical sciences</topic><topic>DNA Restriction Enzymes</topic><topic>DNA Transposable Elements</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation &amp; purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Hybridization</topic><topic>Plasmids</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DYKHUIZEN, D. E</creatorcontrib><creatorcontrib>SAWYER, A. A</creatorcontrib><creatorcontrib>GREEN, L</creatorcontrib><creatorcontrib>MILLER, R. D</creatorcontrib><creatorcontrib>HARTL, D. L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DYKHUIZEN, D. E</au><au>SAWYER, A. A</au><au>GREEN, L</au><au>MILLER, R. D</au><au>HARTL, D. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Joint distribution of insertion elements IS4 and IS5 in natural isolates of Escherichia coli</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>1985-10</date><risdate>1985</risdate><volume>111</volume><issue>2</issue><spage>219</spage><epage>231</epage><pages>219-231</pages><issn>0016-6731</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>A reference collection of natural isolates of Escherichia coli has been studied in order to determine the distribution, abundance and joint occurrence of DNA insertion elements IS4 and IS5. Among these isolates, 36% were found to contain IS4 and 30% were found to contain IS5. Among strains containing IS4 the mean number of copies per strain was 4.4 +/- 0.8; the comparable figure for IS5 was 3.7 +/- 1.0. Although the presence of the elements among the isolates was independent, among those isolates containing both IS4 and IS5, there was a significant negative correlation in the number of copies of the elements. The reference collection was also studied for the presence of the DNA sequences flanking the single copy of IS4 in the chromosome of E. coli K12. Homologous sequences were found in only 26% of the isolates. The sequences flanking the IS4 invariably occur together, and their presence is significantly correlated with the presence of IS4. In eight of the strains that carry these flanking sequences, an IS4 is located between them, and the sequences are present at the homologous position as in the K12 strain. We suggest that IS4 and its flanking sequences share a common mechanism of dissemination, such as plasmids, and we present evidence that they are included in a much larger transposable element.</abstract><cop>Bethesda, MD</cop><pub>Genetics Society of America</pub><pmid>2996976</pmid><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0016-6731
ispartof Genetics (Austin), 1985-10, Vol.111 (2), p.219-231
issn 0016-6731
1943-2631
language eng
recordid cdi_proquest_miscellaneous_76429964
source Freely Accessible Science Journals; Alma/SFX Local Collection
subjects Base Composition
Biological and medical sciences
DNA Restriction Enzymes
DNA Transposable Elements
Escherichia coli
Escherichia coli - genetics
Escherichia coli - isolation & purification
Fundamental and applied biological sciences. Psychology
Genic rearrangement. Recombination. Transposable element
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Plasmids
Species Specificity
title Joint distribution of insertion elements IS4 and IS5 in natural isolates of Escherichia coli
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T17%3A04%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Joint%20distribution%20of%20insertion%20elements%20IS4%20and%20IS5%20in%20natural%20isolates%20of%20Escherichia%20coli&rft.jtitle=Genetics%20(Austin)&rft.au=DYKHUIZEN,%20D.%20E&rft.date=1985-10&rft.volume=111&rft.issue=2&rft.spage=219&rft.epage=231&rft.pages=219-231&rft.issn=0016-6731&rft.eissn=1943-2631&rft.coden=GENTAE&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E76429964%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p181t-b0556197cc45ce1d7200d03401fe425e1e216c5d7c89c5a0c475bb50ca73e71d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=14352759&rft_id=info:pmid/2996976&rfr_iscdi=true