Interaction between human medulloblastomas and foetal rat brain aggregates in vitro

The invasiveness of three medulloblastoma permanent cell lines (D-283, D-341, and DAOY), a human medulloblastoma biopsy, and in addition, a human rhabdomyosarcoma permanent cell line (TE-671), which previously had been regarded as a human medulloblastoma, was studied in an organ co-culture assay. Al...

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Bibliographic Details
Published in:Acta neurochirurgica 1994-01, Vol.126 (1), p.11-16
Main Authors: TERZIS, A. J. A, ARNOLD, H, LAERUM, O.-D, BJERKVIG, R
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biopsy
Brain - pathology
Cell Aggregation - physiology
Cell Line
Cerebellar Neoplasms - pathology
Child
Child, Preschool
Female
Fetus
Humans
Infant
Male
Medical sciences
Medulloblastoma - pathology
Neoplasm Invasiveness - pathology
Neurology
Rats
Rhabdomyosarcoma - pathology
Tumor Stem Cell Assay
Tumors of the nervous system. Phacomatoses
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Summary:The invasiveness of three medulloblastoma permanent cell lines (D-283, D-341, and DAOY), a human medulloblastoma biopsy, and in addition, a human rhabdomyosarcoma permanent cell line (TE-671), which previously had been regarded as a human medulloblastoma, was studied in an organ co-culture assay. All the four cell lines and the biopsy were co-cultured with normal rat brain cell aggregates for up to six days in vitro. The medulloblastoma biopsy, the D-283 and the D-341 cells invaded the brain tissue by diffuse single cell infiltration. The medulloblastoma cell line (DAOY) showed an invasive pattern similar to that observed earlier for most glioblastoma cell lines. This was characterized by massive cell replacement and destruction of normal brain tissue. The rhabdomyosarcoma cell line (TE-671) presented a solid invasive pattern with a fairly well defined border between normal brain and tumour tissue. Thus, the organ co-culture assay system in vitro seems to mimic several aspects of the in situ invasive behaviour of medulloblastomas. It may, therefore, provide new perspectives for pretreatment investigations with chemotherapy and radiotherapy of these malignancies.
ISSN:0001-6268
0942-0940
DOI:10.1007/BF01476488