Epitope mapping human heat shock protein 90 with sera from infected patients

Epitope mapping with sera from a range of infected patients showed that antibodies are commonly produced which cross‐react with a number of epitopes on human heat shock protein 90 (HSP 90). Such autoreactive antibodies were particularly frequent in patients suffering from systemic candidiasis (9 pat...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 1994-01, Vol.8 (1), p.43-48
Main Authors: Al‐Dughaym, A.M., Matthews, R.C., Burnie, J.P.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antibody Specificity
Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation
Aspergillus
Aspergillus infection
Autoantibodies - blood
Autoantibodies - immunology
Bacterial Infections - blood
Bacterial Infections - immunology
Biological and medical sciences
Candida
Cross Reactions
Disseminated candidiasis
Epitope mapping
Epitopes - chemistry
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Heat Shock Protein 90
Heat-Shock Proteins - chemistry
Heat-Shock Proteins - immunology
Humans
Lupus Erythematosus, Systemic - blood
Lupus Erythematosus, Systemic - immunology
Malaria
Malaria - blood
Malaria - immunology
Molecular immunology
Molecular Sequence Data
Mycoses - blood
Mycoses - immunology
Plasmodium
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Summary:Epitope mapping with sera from a range of infected patients showed that antibodies are commonly produced which cross‐react with a number of epitopes on human heat shock protein 90 (HSP 90). Such autoreactive antibodies were particularly frequent in patients suffering from systemic candidiasis (9 patients), invasive aspergillosis (6 patients), ABPA (2 patients), a patient with aspergilloma and one with malaria. The patient with malaria recognised similar epitopes to those with invasive aspergillosis and candidiasis including the highly conserved epitope LKVIRKVIRK and an epitope NNLGTI which was otherwise only recognised by patients with candidiasis. Crossreacting antibodies to relatively few epitopes occurred in patients with Enterococcus faecalis and Corynebacterium jeikeium endocarditis. This was contrasted with the results from 6 patients with systemic lupus erythematosus (SLE) who were positive on immunoblot against fungal HSP 90. These did not react with the above epitopes but reacted with other areas within human HSP 90.
ISSN:0928-8244
1574-695X
DOI:10.1111/j.1574-695X.1994.tb00423.x