Incomplete Neutralization of Hepatitis A Virus in vitro due to Lipid-associated Virions

547 Burnett-Womack CSB, 229H, Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514 and 1 Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, D.C., U.S.A. Hepatitis A virus (HAV) released...

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Published in:Journal of general virology 1985-11, Vol.66 (11), p.2501-2505
Main Authors: Lemon, Stanley M, Binn, Leonard N
Format: Article
Language:English
Subjects:
Animals
antisera
Biological and medical sciences
Cell Line
Cell Membrane - physiology
cell membranes
Centrifugation, Density Gradient
Cercopithecus aethiops
Chloroform
Fundamental and applied biological sciences. Psychology
Hepatitis A Antibodies
hepatitis A virus
Hepatitis Antibodies - immunology
Hepatovirus - immunology
Hepatovirus - metabolism
Humans
Immune Sera - immunology
Lipid Metabolism
lipids
Lipids - physiology
Microbiology
neutralization
Neutralization Tests
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Virion - metabolism
virions
Virology
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Summary:547 Burnett-Womack CSB, 229H, Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514 and 1 Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, D.C., U.S.A. Hepatitis A virus (HAV) released from infected BS-C-1 cells was incompletely neutralized when incubated with a variety of convalescent sera (non-neutralizable fraction of 17 to 32%). Chloroform extraction of virus resulted in a substantial reduction of the non-neutralizable fraction (to less than 1%), suggesting that nonneutralizable virions might be associated with lipids. Non-neutralizable HAV recovered from untreated cell culture supernatant fluids sedimented heterogeneously and less rapidly than normal virus in rate-zonal sucrose gradients and also banded at a lower density in CsCl (1.14 to 1.18 g/ml) than normal, neutralizable virus (1.32 g/ml). This bimodal distribution of HAV in CsCl gradients was confirmed by cDNA-RNA hybridization. Together, these observations suggest that a substantial proportion of HAV particles released from infected cells are lipid-associated and imply an important role for cell membranes in the assembly and release of HAV in vitro . Keywords: HAV, neutralization, chloroform Received 12 March 1985; accepted 25 July 1985.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-66-11-2501