Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice

We determined whether Borrelia burgdorferi outer surface proteins (Osps) E and F could elicit immune responses useful for a Lyme disease vaccine. Thirty days after challenge with B. burgdorferi, mice produced antibodies to OspE but not OspF, whereas antibodies to OspF were present in sera of mice ob...

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Bibliographic Details
Published in:Infection and Immunity 1994-05, Vol.62 (5), p.2079-2084
Main Authors: Nguyen, T.P.K, Lam, T.T, Barthold, S.W, Telford, S.R. III, Flavell, R.A, Fikrig, E
Format: Article
Language:English
Subjects:
Acari
Animals
ANTIGENE
ANTIGENOS
Antigens, Bacterial - immunology
Bacterial Outer Membrane Proteins - immunology
Bacterial Proteins
Bacteriology
Base Sequence
Biological and medical sciences
BORRELIA BURGDORFERI
Borrelia burgdorferi Group - immunology
Female
Fundamental and applied biological sciences. Psychology
GENERO HUMANO
GENRE HUMAIN
Humans
IMMUNITE HUMORALE
Immunization
INMUNIDAD HUMORAL
IXODES
Ixodidae
Lipoproteins
Mice
Mice, Inbred C3H
Microbiology
Molecular Sequence Data
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Rabbits
RATON
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
SOURIS
Ticks - microbiology
VACCIN
VACUNA
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Summary:We determined whether Borrelia burgdorferi outer surface proteins (Osps) E and F could elicit immune responses useful for a Lyme disease vaccine. Thirty days after challenge with B. burgdorferi, mice produced antibodies to OspE but not OspF, whereas antibodies to OspF were present in sera of mice obtained 90 days after infection. Examination of sera from patients with Lyme disease revealed antibodies to OspF in a small number (14%) of early-stage disease patients but in a majority (58%) of patients with late-stage disease, while antibodies to OspE were rarely detected in patients. Mice immunized with recombinant OspE or OspF produced high titers of antibodies to OspE or OspF, respectively. OspF-immunized mice were partially protected from both intradermal syringe challenge and tick-mediated transmission of B. burgdorferi while vaccination with OspE did not confer immunity B. burgdorferi organisms were, however, substantially destroyed within ticks that engorged on either OspE-(75% reduction in the number of spirochetes within the ticks, compared with controls) or OspF (90% reduction in the number of spirochetes within the ticks)-immunized mice
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.62.5.2079-2084.1994