Microtiter plate adherence assay and receptor analogs for Mycoplasma hyopneumoniae

A microliter plate adherence assay for Mycoplasma hyopneumoniae was established by use of purified swine tracheal cilia which contained receptors for the mycoplasmas. M. hyopneumoniae bound specifically to plates coated with solubilized cilia. The binding was dependent on both the concentration of c...

Full description

Saved in:
Bibliographic Details
Published in:Infection and Immunity 1994-05, Vol.62 (5), p.1616-1622
Main Authors: Zhang, Q, Young, T.F, Ross, R.F
Format: Article
Language:English
Subjects:
Animals
Bacterial Adhesion - drug effects
Bacteriology
Biological and medical sciences
CERDO
CHIMIORECEPTEUR
Cilia - microbiology
ESTRUCTURA CELULAR
Fundamental and applied biological sciences. Psychology
METHODE D'ESSAI
METODOS DE ENSAYO
Microbiology
Mycoplasma - pathogenicity
MYCOPLASMA HYOPNEUMONIAE
Neuraminidase - pharmacology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
PORCIN
PROPIEDADES MECANICAS
PROPRIETE MECANIQUE
QUIMIORECEPTORS
Receptors, Cell Surface - analysis
STRUCTURE CELLULAIRE
Swine
Trachea - microbiology
TRACHEE
TRAQUEA
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A microliter plate adherence assay for Mycoplasma hyopneumoniae was established by use of purified swine tracheal cilia which contained receptors for the mycoplasmas. M. hyopneumoniae bound specifically to plates coated with solubilized cilia. The binding was dependent on both the concentration of cilia and the number of mycoplasmas. Dextran sulfate, heparin, chondroitin sulfate, laminin, mucin, and fucoidan significantly inhibited the binding of the mycoplasmas. The six inhibitors also disrupted the adherence of the mycoplasmas to intact ciliated cells. Preincubation with either mycoplasmas or cilia indicated that heparin, mucin, fucoidan, and chondroitin sulfate interacted with the adhesive molecules on the surface of the mycoplasmas, while laminin blocked the receptors in cilia. The basis for the inhibition induced by dextran sulfate was unknown. Treatment of cilia with neuraminidase appeared to promote adherence of the mycoplasmas, whereas treatment of cilia with sodium metaperiodate decreased binding. These results indicate that receptors for M. hyopneumoniae in the ciliated epithelium of the respiratory tract of pigs are glycoconjugate in nature
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.62.5.1616-1622.1994