Specific Diagnosis of Progressive Multifocal Leukoencephalopathy by Polymerase Chain Reaction

Using polymerase chain reaction (PCR), 34 cerebrospinal fluid (CSF) samples from 28 patients with progressive multifocal leukoencephalopathy (PML) were analyzed. As controls, 116 samples were evaluated from 82 human immunodeficiency virus type 1 (HIV-1)-infected patients and 1 HIV-1-negative patient...

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Bibliographic Details
Published in:The Journal of infectious diseases 1994-05, Vol.169 (5), p.1138-1141
Main Authors: Weber, Thomas, Turner, Rodney W., Frye, Stephan, Ruf, Bernhard, Haas, Judith, Schielke, Eva, Pohle, Hans-Dieter, Lüke, Wolfgang, Lüer, Wilfried, Felgenhauer, Klaus, Hunsmann, Gerhard
Format: Article
Language:English
Subjects:
AIDS/HIV
Bacteria
Bacterial infections
Base Sequence
Biological and medical sciences
Blood
Concise Communications
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
DNA, Viral
Enterotoxins
HIV Infections - complications
Humans
Infections
JC virus
Kidneys
Leukoencephalopathy, Progressive Multifocal - cerebrospinal fluid
Leukoencephalopathy, Progressive Multifocal - complications
Leukoencephalopathy, Progressive Multifocal - diagnosis
Medical sciences
Molecular Sequence Data
Neurology
Polymerase Chain Reaction
Polyomavirus - genetics
Polyomavirus - isolation & purification
Sensitivity and Specificity
Staphylococcus
Superantigens
T lymphocytes
Toxins
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Summary:Using polymerase chain reaction (PCR), 34 cerebrospinal fluid (CSF) samples from 28 patients with progressive multifocal leukoencephalopathy (PML) were analyzed. As controls, 116 samples were evaluated from 82 human immunodeficiency virus type 1 (HIV-1)-infected patients and 1 HIV-1-negative patient. Of the HIV-1-positive patients, 23 had cerebral toxoplasmosis, 10 had HIV leukoencephalopathy, and 49 had other neurologic complications. Detection of JC virus (JCV) DNA in CSF was increased 10-fold by the addition of carrier DNA before phenol-chloroform-isoamyl alcohol extraction. The primer pair JC 26/29, from the VP1/large T region, had a limit of detection of 105 JCV DNA molecules/100 µL. The primer pair JC 36/39, located in the large T gene region, had a 100-fold lower limit of detection. With JC 26/29, the sensitivity was 43% (12/28) and specificity was 100%. Using JC 36/39, sensitivity increased to 82% (23/28), and false-positive results were not observed. Diagnosis of PML is greatly aided by PCR analysis of CSF.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/169.5.1138