Influence of feed intake and starvation on the magnitude of Na+,K+-ATPase(EC 3.6.1.3)-dependent respiration in duodenal mucosa of sheep

• Published in: British journal of nutrition 1985-05, Vol.53 (3), p.605-614
• Main Authors: McBride, B. W., Milligan, L. P.
• Format: Article
• Language: English
• Subjects: adenosinetriphosphatase; Animals; Biological and medical sciences; Dose-Response Relationship, Drug; duodenum; Duodenum - metabolism; feed intake; Food; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Intestine. Mesentery; Male; mucosa; Ouabain - pharmacology; Oxygen Consumption; Papers on General Nutrition; respiration; Rubidium - metabolism; Sheep; Sodium-Potassium-Exchanging ATPase - physiology; starvation; Starvation - metabolism; Vertebrates: digestive system
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1079/BJN19850070

1. Oxygen consumption and Na+,K+-ATPase(EC 3.6.1.3)-dependent (ouabain-sensitive) and -independent respiration were measured for duodenal mucosa biopsies from 10-month-old sheep given two levels of digestible energy (DE) intake (7.6–7.7 and 14.8 MJ lucerne (Medicago sativa) pellets/d) and following 48 h of starvation. 2. The mucosal biopsies were determined to be structurally intact and free of adherent bacteria on histological and scanning-electron-microscope examinations. 3. The use of D-glucose as a substrate during incubations did not elevate (P > 0.05) the respiration indices of the biopsies over those measured during acetate incubations. 4. Glucose uptake did not (P > 0.05) influence the Na+,K+-ATPase-dependent respiration of the mucosal biopsies. 5. Na+,K+-ATPase-dependent respiration accounted for 50% of the total O2, consumption of the mucosal biopsies of sheep given the lower level of DE. 6. Total O2, consumption of the duodenalmucosa was not (P > 0.05) increased when sheep were given the higher level of DE but Na+,K+-ATPase-dependent respiration of the mucosa was elevated (P < 0.01) by 37% during this period. 7. When sheep were starved for 48 h, total O2, consumption of the mucosal biopsies was not (P > 0.05) affected, however, Na+,K+-ATPase-dependent respiration of the biopsies dropped (P < 0.01) by 45%. 8. Na+,K+-ATPase-dependknt respiration accounted for 61.3% of the O2, uptakes of mucosa from the sheep given the higher level of DE and 28.3% of the 02, uptake of mucosa from fasted sheep.