A double stable isotope technique for measuring iron absorption in infants

A stable isotope technique has been developed which uses 57Fe and 58Fe as labels and which enables the simultaneous measurement of Fe absorption from two test meals in infants. The method was evaluated by measuring Fe absorption from a commercial whey-adjusted infant formula in nine healthy infants...

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Bibliographic Details
Published in:British journal of nutrition 1994-03, Vol.71 (3), p.411-424
Main Authors: Kastenmayer, Peter, Davidsson, Lena, Galanz, Pilar, Cherouvrier, Françise, Hercberg, Serge, Hurrell, Richard F.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Child, Preschool
Female
Fundamental and applied biological sciences. Psychology
Humans
Infant
Infant Food
Infants
Intestinal Absorption
Intestine. Mesentery
Iron
Iron - metabolism
iron absorption
Iron Isotopes
Male
Mass Spectrometry
measurement
methodology
Stable isotopes
Thermal ionization mass spectrometry
Vertebrates: digestive system
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Summary:A stable isotope technique has been developed which uses 57Fe and 58Fe as labels and which enables the simultaneous measurement of Fe absorption from two test meals in infants. The method was evaluated by measuring Fe absorption from a commercial whey-adjusted infant formula in nine healthy infants aged 13–25 weeks. Each infant was fed 210 ml formula, labelled with either 57Fe or 58Fe, on four consecutive mornings, in random order. The total Fe content in each feed was 2.5 mg Fe; either as 2.5 mg 57Fe, or 0 6 mg 58Fe plus 1.9 mg Fe with normal isotopic composition. Isotopic enrichment of Fe in erythrocytes was measured by thermal ionization mass spectrometry 14 d after the last administration, and Fe absorption was calculated based on isotope ratio shifts, total circulating Fe and intake of each isotope. Geometric mean absorption for the 57Fe and 58Fe labels was 6.72 and 658% respectively, and the absorption of the two isotopes was not significantly different (Student's paired t test). By this technique, paired comparisons of Fe absorption can be obtained and systematic studies of the influence of dietary factors on Fe absorption during infancy can he conducted.
ISSN:0007-1145
1475-2662
DOI:10.1079/BJN19940148