Identification of a novel growth factor-responsive gene in vascular smooth muscle cells

We have employed differential screening of a rat aortic smooth muscle cell cDNA library to isolate a cDNA clone, SM-20, whose nucleotide and deduced amino acid sequences are distinct from those reported previously. SM-20 encodes an mRNA of approximately 2.5-3.0 kilobase pairs which is present at low...

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-04, Vol.269 (17), p.13041-13047
Main Authors: WAX, S. D, ROSENFIELD, C.-L, TAUBMAN, M. B
Format: Article
Language:English
Subjects:
3T3 Cells
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cells, Cultured
Cloning, Molecular
DNA, Complementary
DNA-Binding Proteins
Fundamental and applied biological sciences. Psychology
Genes. Genome
Growth Substances - physiology
Hypoxia-Inducible Factor-Proline Dioxygenases
Immediate-Early Proteins - genetics
Immediate-Early Proteins - metabolism
Male
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Muscle, Smooth, Vascular - metabolism
Procollagen-Proline Dioxygenase
Rats
Rats, Sprague-Dawley
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
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Summary:We have employed differential screening of a rat aortic smooth muscle cell cDNA library to isolate a cDNA clone, SM-20, whose nucleotide and deduced amino acid sequences are distinct from those reported previously. SM-20 encodes an mRNA of approximately 2.5-3.0 kilobase pairs which is present at low levels in quiescent vascular smooth muscle cells. SM-20 levels increase within 1 h of treatment with growth agonists (serum, platelet-derived growth factor, angiotensin II), isoproterenol, and forskolin. Cycloheximide induces high levels of SM-20 mRNA and superinduces it in the presence of serum, suggesting that the increase in SM-20 mRNA levels is not dependent on protein synthesis and is part of the primary response to growth agonists. SM-20 is expressed at high levels in tissues and cells derived from muscle (smooth, skeletal, and cardiac) and nerve (brain, PC12 cells) and is not expressed in 3T3 fibroblasts or rat 6 fibroblasts. SM-20 encodes a new member of the immediate early gene family and is unusual in that it is expressed in vascular smooth muscle, but not in fibroblasts.
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(18)99981-3