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Identification of a novel growth factor-responsive gene in vascular smooth muscle cells
We have employed differential screening of a rat aortic smooth muscle cell cDNA library to isolate a cDNA clone, SM-20, whose nucleotide and deduced amino acid sequences are distinct from those reported previously. SM-20 encodes an mRNA of approximately 2.5-3.0 kilobase pairs which is present at low...
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| Published in: | The Journal of biological chemistry 1994-04, Vol.269 (17), p.13041-13047 |
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| container_end_page | 13047 |
| container_issue | 17 |
| container_start_page | 13041 |
| container_title | The Journal of biological chemistry |
| container_volume | 269 |
| creator | WAX, S. D ROSENFIELD, C.-L TAUBMAN, M. B |
| description | We have employed differential screening of a rat aortic smooth muscle cell cDNA library to isolate a cDNA clone, SM-20, whose
nucleotide and deduced amino acid sequences are distinct from those reported previously. SM-20 encodes an mRNA of approximately
2.5-3.0 kilobase pairs which is present at low levels in quiescent vascular smooth muscle cells. SM-20 levels increase within
1 h of treatment with growth agonists (serum, platelet-derived growth factor, angiotensin II), isoproterenol, and forskolin.
Cycloheximide induces high levels of SM-20 mRNA and superinduces it in the presence of serum, suggesting that the increase
in SM-20 mRNA levels is not dependent on protein synthesis and is part of the primary response to growth agonists. SM-20 is
expressed at high levels in tissues and cells derived from muscle (smooth, skeletal, and cardiac) and nerve (brain, PC12 cells)
and is not expressed in 3T3 fibroblasts or rat 6 fibroblasts. SM-20 encodes a new member of the immediate early gene family
and is unusual in that it is expressed in vascular smooth muscle, but not in fibroblasts. |
| doi_str_mv | 10.1016/s0021-9258(18)99981-3 |
| format | article |
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nucleotide and deduced amino acid sequences are distinct from those reported previously. SM-20 encodes an mRNA of approximately
2.5-3.0 kilobase pairs which is present at low levels in quiescent vascular smooth muscle cells. SM-20 levels increase within
1 h of treatment with growth agonists (serum, platelet-derived growth factor, angiotensin II), isoproterenol, and forskolin.
Cycloheximide induces high levels of SM-20 mRNA and superinduces it in the presence of serum, suggesting that the increase
in SM-20 mRNA levels is not dependent on protein synthesis and is part of the primary response to growth agonists. SM-20 is
expressed at high levels in tissues and cells derived from muscle (smooth, skeletal, and cardiac) and nerve (brain, PC12 cells)
and is not expressed in 3T3 fibroblasts or rat 6 fibroblasts. SM-20 encodes a new member of the immediate early gene family
and is unusual in that it is expressed in vascular smooth muscle, but not in fibroblasts.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(18)99981-3</identifier><identifier>PMID: 8175725</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>3T3 Cells ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cells, Cultured ; Cloning, Molecular ; DNA, Complementary ; DNA-Binding Proteins ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Growth Substances - physiology ; Hypoxia-Inducible Factor-Proline Dioxygenases ; Immediate-Early Proteins - genetics ; Immediate-Early Proteins - metabolism ; Male ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Muscle, Smooth, Vascular - metabolism ; Procollagen-Proline Dioxygenase ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid</subject><ispartof>The Journal of biological chemistry, 1994-04, Vol.269 (17), p.13041-13047</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-377d5b7ebf2366be38126ba409eebb924e382f2c112f347acf6c9efd34a5bd8e3</citedby><cites>FETCH-LOGICAL-c506t-377d5b7ebf2366be38126ba409eebb924e382f2c112f347acf6c9efd34a5bd8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4153839$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8175725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WAX, S. D</creatorcontrib><creatorcontrib>ROSENFIELD, C.-L</creatorcontrib><creatorcontrib>TAUBMAN, M. B</creatorcontrib><title>Identification of a novel growth factor-responsive gene in vascular smooth muscle cells</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>We have employed differential screening of a rat aortic smooth muscle cell cDNA library to isolate a cDNA clone, SM-20, whose
nucleotide and deduced amino acid sequences are distinct from those reported previously. SM-20 encodes an mRNA of approximately
2.5-3.0 kilobase pairs which is present at low levels in quiescent vascular smooth muscle cells. SM-20 levels increase within
1 h of treatment with growth agonists (serum, platelet-derived growth factor, angiotensin II), isoproterenol, and forskolin.
Cycloheximide induces high levels of SM-20 mRNA and superinduces it in the presence of serum, suggesting that the increase
in SM-20 mRNA levels is not dependent on protein synthesis and is part of the primary response to growth agonists. SM-20 is
expressed at high levels in tissues and cells derived from muscle (smooth, skeletal, and cardiac) and nerve (brain, PC12 cells)
and is not expressed in 3T3 fibroblasts or rat 6 fibroblasts. SM-20 encodes a new member of the immediate early gene family
and is unusual in that it is expressed in vascular smooth muscle, but not in fibroblasts.</description><subject>3T3 Cells</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>DNA-Binding Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Growth Substances - physiology</subject><subject>Hypoxia-Inducible Factor-Proline Dioxygenases</subject><subject>Immediate-Early Proteins - genetics</subject><subject>Immediate-Early Proteins - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Procollagen-Proline Dioxygenase</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNqFkEtv1DAQgC0EKkvhJ1TyASE4BDx24sexWhWoVIkDILhZjjPeNUrixU624t-T0NX2yFxGmvnmoY-QK2DvgYH8UBjjUBne6Leg3xljNFTiCdkA06ISDfx8SjZn5Dl5UcovtkRt4IJcaFCN4s2G_LjtcJxiiN5NMY00BeromI7Y011O99OeBuenlKuM5ZDGEo9IdzgijSM9uuLn3mVahpQWcpiL75F67PvykjwLri_46pQvyfePN9-2n6u7L59ut9d3lW-YnCqhVNe0CtvAhZQtCg1ctq5mBrFtDa-XCg_cA_AgauV8kN5g6ETtmrbTKC7Jm4e9h5x-z1gmO8SyfuBGTHOxStYKhJL_BUFqqQUXC9g8gD6nUjIGe8hxcPmPBWZX8_brqtWuWi1o-8-8XeeuTgfmdsDuPHVSvfRfn_qLNteH7EYfyxmroRFamEdsH3f7-5jRtjH5PQ6WS2NBWRCsBvEXOKiYog</recordid><startdate>19940429</startdate><enddate>19940429</enddate><creator>WAX, S. 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B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-377d5b7ebf2366be38126ba409eebb924e382f2c112f347acf6c9efd34a5bd8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>3T3 Cells</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>DNA-Binding Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Growth Substances - physiology</topic><topic>Hypoxia-Inducible Factor-Proline Dioxygenases</topic><topic>Immediate-Early Proteins - genetics</topic><topic>Immediate-Early Proteins - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Procollagen-Proline Dioxygenase</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WAX, S. D</creatorcontrib><creatorcontrib>ROSENFIELD, C.-L</creatorcontrib><creatorcontrib>TAUBMAN, M. 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nucleotide and deduced amino acid sequences are distinct from those reported previously. SM-20 encodes an mRNA of approximately
2.5-3.0 kilobase pairs which is present at low levels in quiescent vascular smooth muscle cells. SM-20 levels increase within
1 h of treatment with growth agonists (serum, platelet-derived growth factor, angiotensin II), isoproterenol, and forskolin.
Cycloheximide induces high levels of SM-20 mRNA and superinduces it in the presence of serum, suggesting that the increase
in SM-20 mRNA levels is not dependent on protein synthesis and is part of the primary response to growth agonists. SM-20 is
expressed at high levels in tissues and cells derived from muscle (smooth, skeletal, and cardiac) and nerve (brain, PC12 cells)
and is not expressed in 3T3 fibroblasts or rat 6 fibroblasts. SM-20 encodes a new member of the immediate early gene family
and is unusual in that it is expressed in vascular smooth muscle, but not in fibroblasts.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8175725</pmid><doi>10.1016/s0021-9258(18)99981-3</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1994-04, Vol.269 (17), p.13041-13047 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | 3T3 Cells Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cells, Cultured Cloning, Molecular DNA, Complementary DNA-Binding Proteins Fundamental and applied biological sciences. Psychology Genes. Genome Growth Substances - physiology Hypoxia-Inducible Factor-Proline Dioxygenases Immediate-Early Proteins - genetics Immediate-Early Proteins - metabolism Male Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Muscle, Smooth, Vascular - metabolism Procollagen-Proline Dioxygenase Rats Rats, Sprague-Dawley RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid |
| title | Identification of a novel growth factor-responsive gene in vascular smooth muscle cells |
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