Conjugative transfer of tetracycline resistance in rumen streptococcal strains

In 11% of tested Streptococcus bovis strains a conjugative transfer of tetracycline resistance was observed when mating experiments were carried out on membrane filters. The recipient strain used was S. bovis BM114 with chromosomal resistance to rifampicin. In addition, in two strains tetracycline r...

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Bibliographic Details
Published in:Folia microbiologica 1994-01, Vol.39 (1), p.83-86
Main Authors: Jonecova, Z. (Ustav Experimentalnej Veterinarnej Mediciny, Kosice (Slovak Republic)), Marekova, M, Kmet, V
Format: Article
Language:English
Subjects:
ADN
AMILOPECTINA
AMYLOPECTIN
AMYLOPECTINE
Animals
Bacteriology
Biological and medical sciences
CARBON
CARBONE
CARBONO
CHEMICAL RESISTANCE
Conjugation, Genetic
DNA
Enterococcus faecium - genetics
Fundamental and applied biological sciences. Psychology
Genetics
GLUCOSA
GLUCOSE
MALTOSA
MALTOSE
Microbiology
PLASMIDE
PLASMIDIOS
PLASMIDS
R Factors - genetics
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTENCIA QUIMICA
RUMEN
Rumen - microbiology
STREPTOCOCCUS BOVIS
Streptococcus bovis - drug effects
Streptococcus bovis - genetics
Streptococcus bovis - isolation & purification
TETRACICLINA
TETRACYCLINE
Tetracycline Resistance - genetics
TETRACYCLINES
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Summary:In 11% of tested Streptococcus bovis strains a conjugative transfer of tetracycline resistance was observed when mating experiments were carried out on membrane filters. The recipient strain used was S. bovis BM114 with chromosomal resistance to rifampicin. In addition, in two strains tetracycline resistance was transferred also to recipient strain Enterococcus faecium AL6. The transfer frequencies were in the range of 10(-6) to 10(-3). The donor strains were screened for the presence of plasmids and one up to four bands of plasmid DNA in all tested strains were revealed. In spite of that isolation of plasmid DNA was successful only in 53/4/114 transconjugants. Transconjugant 32/114 contained amylase activity which was higher than in the donor strain.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02814537