Rat CINC/gro: a novel mediator for locomotive and secretagogue activation of neutrophils in vivo

• Published in: Journal of leukocyte biology 1994-05, Vol.55 (5), p.652-657
• Main Authors: Suzuki, Hidekazu, Suematsu, Makoto, Miura, Soichiro, Liu, Yu Ying, Watanabe, Kazuyoshi, Miyasaka, Masayuki, Tsurufuji, Susumu, Tsuchiya, Masaharu
• Format: Article
• Language: English
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies, Monoclonal; Biological and medical sciences; Cell Adhesion - physiology; Cell Communication - physiology; Cell Movement - physiology; Chemokine CXCL1; Chemokines, CXC; Chemotactic Factors - pharmacology; Chemotactic Factors - physiology; Endothelium, Vascular - cytology; Endothelium, Vascular - physiology; free; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Growth Substances - pharmacology; Growth Substances - physiology; Immunobiology; Intercellular Signaling Peptides and Proteins; interleukin‐8; Leukocytes - cytology; Leukocytes - physiology; Luminescent Measurements; Lymphokines, interleukins ( function, expression); Male; microcirculation; Neutrophils - cytology; Neutrophils - physiology; radical; Rats; Rats, Wistar; Regulatory factors and their cellular receptors; β2‐integrin
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.55.5.652

The effects of rat CINC/gro, a member of the interleukin‐8 family, on the endothelium‐neutrophil interaction and transendothelial macromolecular leakage were studied in rat mesenteric microcirculation. Thirty minutes after superfusion with 10 nM CINC/gro, the number of neutrophils adherent to the venular endothelium and those migrated across the venules were significantly increased with a concomitant elevation of luminol‐dependent chemiluminescence at the site of adhesion. Transendothelial macromolecular leakage as assessed by the relative length of venular wall stained with monastral blue B was also increased at 30 min after the start of CINC/gro superfusion. Pretreatments with a CD18‐directed monoclonal antibody, WT‐3 (1 mg/kg), significantly attenuated the increase in number of adherent and migrated neutrophils, the increase in luminol‐dependent chemiluminescence, and the venular macromolecular leakage after the application of CINC/gro. These data suggest that CINC/gro is a novel stimulator that evokes not only locomotive but also secretagogue activation of neutrophils via a CD18‐dependent mechanism in viva J. Leukoc. Biol. 55: 652–657; 1994.