A constitutively transcribed actin gene is associated with the nuclear matrix in a Drosophila cell line

The relationship between transcriptional activity and gene association with the nuclear matrix has been investigated in Drosophila melanogaster. The nuclear matrix of Schneider cell line 2 of Drosophila was isolated and observed to conform to expected dimensions in phase contrast and scanning electr...

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Bibliographic Details
Published in:In vitro cellular & developmental biology 1985-11, Vol.21 (11), p.641-648
Main Authors: Intres, R, Donady, J.J
Format: Article
Language:English
Subjects:
ACTIN
Actins - genetics
Actins - isolation & purification
Animals
Biological and medical sciences
Cell nucleus
Cell Nucleus - metabolism
Cell Nucleus - ultrastructure
Cell structures and functions
CULTIVO DE TEJIDOS
CULTURE DE TISSUS
Cytoplasm - metabolism
DNA Replication
DROSOPHILA
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Fundamental and applied biological sciences. Psychology
GENE
GENES
Kinetics
Microscopy, Electron, Scanning
Microscopy, Phase-Contrast
Molecular and cellular biology
Molecular Weight
Myosins - genetics
NOYAU CELLULAIRE
NUCLEO
NUCLEUS
PROTEINAS
PROTEINE
PROTEINS
TISSUE CULTURE
Transcription, Genetic
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Summary:The relationship between transcriptional activity and gene association with the nuclear matrix has been investigated in Drosophila melanogaster. The nuclear matrix of Schneider cell line 2 of Drosophila was isolated and observed to conform to expected dimensions in phase contrast and scanning electron microscopic preparations. This structure contains proteins that appear similar to the intact nucleus. High salt extracted nuclei digested with DNase I released 98% of the DNA, whereas digestion with Eco RI released a maximum of 80%. These and other nuclease digestions indicate that satellite DNA as well as some unique sequence DNA are bound to the nuclear matrix. A constitutively transcribed actin gene was enriched in the nuclear matrix bound DNA. Two other nontranscribed genes, a muscle-specific actin gene and the myosin heavy chain gene, showed no enrichment in nuclear matrix DNA.
ISSN:0883-8364
2327-431X