13-Hydroxyoctadecadienoic acid is the vessel wall chemorepellant factor, LOX

We have previously reported that endothelial cells synthesize a cytosol-associated, lipoxygenase-derived metabolite, LOX, which acts as a chemorepellant and, in so doing, maintains the vessel wall thromboresistance. In this study we demonstrate that LOX is a 13-hydroxylinoleic acid (13-OH-18:2) deri...

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Bibliographic Details
Published in:The Journal of biological chemistry 1985-12, Vol.260 (30), p.16056-16059
Main Authors: Buchanan, M R, Haas, T A, Lagarde, M, Guichardant, M
Format: Article
Language:English
Subjects:
Antithrombins
Arachidonic Acid
Arachidonic Acids - metabolism
Carbon Radioisotopes
Cells, Cultured
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Female
Fibroblasts - metabolism
Gas Chromatography-Mass Spectrometry
Humans
Linoleic Acid
Linoleic Acids - biosynthesis
Linoleic Acids - isolation & purification
Linoleic Acids - metabolism
Lipoxygenase - metabolism
Muscle, Smooth, Vascular - metabolism
Pregnancy
Umbilical Veins - metabolism
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Summary:We have previously reported that endothelial cells synthesize a cytosol-associated, lipoxygenase-derived metabolite, LOX, which acts as a chemorepellant and, in so doing, maintains the vessel wall thromboresistance. In this study we demonstrate that LOX is a 13-hydroxylinoleic acid (13-OH-18:2) derived from linoleic acid and identical to 13-hydroxy-9-cis,11-trans-octadecadienoic acid, as measured by both reverse phase high pressure liquid chromatography and gas chromatography/mass spectrometry. In addition, we demonstrate that 13-OH-18:2 is produced in significantly greater quantities by endothelial cells than by smooth muscle cels or by fibroblasts. Furthermore, we demonstrate that 13-OH-18:2 is produced in microgram amounts under basal conditions and is decreased by thrombin, calcium ionophore, and trypsin stimulation. And finally, we demonstrate that endothelial cells do not synthesize any significant amounts of lipoxygenase-derived arachidonic acid metabolites either under basal or stimulated conditions unless exogenous arachidonic acid is added. These observations indicate that the major lipoxygenase-derived, chemorepellant metabolite produced by the endothelial is 13-hydroxy-9-cis,11-trans-octadecadienoic acid.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)36198-7