Identification of neisseria gonorrhoeae in synovial fluid using the polymerase chain reaction

• Published in: Arthritis and rheumatism 1994-05, Vol.37 (5), p.702-709
• Main Authors: Liebling, Michael R., Arkfeld, Daniel G., Michelini, Giulia A., Nishio, Midori J., Eng, Bett J., Jin, Tian, Louie, James S.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Arthritis, Infectious - diagnosis; Arthritis, Reactive - diagnosis; Bacterial arthritis and osteitis; Bacterial diseases; Base Sequence; Biological and medical sciences; Diagnosis, Differential; DNA, Bacterial - analysis; Female; Gonorrhea - diagnosis; Human bacterial diseases; Humans; Infectious diseases; Male; Medical sciences; Middle Aged; Molecular Sequence Data; Neisseria gonorrhoeae; Neisseria gonorrhoeae - genetics; Neisseria gonorrhoeae - isolation & purification; Polymerase Chain Reaction; Predictive Value of Tests; Sensitivity and Specificity; Synovial Fluid - microbiology
• ISSN: 0004-3591; 1529-0131
• DOI: 10.1002/art.1780370514

Objective. To analyze synovial fluid (SF) for the presence of Neisseria gonorrhoeae DNA using the polymerase chain reaction (PCR). Methods. We used a modified, nested PCR to detect the presence of N gonorrhoeae DNA in 41 samples of SF obtained from 10 patients with clinical gonococcal arthritis whose SF samples were sterile by culture and from 27 controls, including 11 patients with Reiter's syndrome. Results obtained using this method were compared with those obtained using the GEN‐PROBE system, an RNA–DNA hybridization technique. Results. With nested PCR, N gonorrhoeae DNA was detected in 11 of 14 SF samples obtained from patients with culture‐negative clinical gonococcal arthritis but in none of the 11 SF samples from Reiter's syndrome patients. The specificity of this technique was 96.4%, with a sensitivity of 78.6%. The rate of false‐positive results was 3.6%. The GEN‐PROBE technique was unable to detect N gonorrhoeae ribosomal RNA in any of the samples. Conclusion. These findings demonstrate the potential utility of the PCR in confirming the clinical diagnosis of gonococcal arthritis as well as providing insight into the pathogenesis of this disorder in patients whose SF are sterile by standard culture techniques. PCR may also prove helpful in differentiating N gonorrhoeae arthritis from acute Reiter's syndrome.