Dietary restriction from middle age attenuates age-associated lymphoma development and interleukin 6 dysregulation in C57BL/6 mice

Dietary restriction (DR) started in middle age profoundly reduces the occurrence of lymphoma in C57BL/6 mice. Here, we report immunocellular and molecular changes associated with this mode of cancer prevention. Twelve-month-old male C57BL/6 mice were either fed a control diet or subjected to moderat...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1994-06, Vol.54 (11), p.3054-3061
Main Authors: VOLK, M. J, PUGH, T. D, MOONJU KIM, FRITH, C. H, DAYNES, R. A, ERSHLER, W. B, WEINDRUCH, R
Format: Article
Language:English
Subjects:
Age Factors
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Body Weight
Diet
Experimental malignant blood diseases
Incidence
Interleukin-6 - blood
Leukocyte Count
Lymphocyte Subsets
Lymphocytes
Lymphoma - blood
Lymphoma - epidemiology
Lymphoma - mortality
Lymphoma - prevention & control
Male
Medical sciences
Mice
Mice, Inbred C57BL
Proto-Oncogene Proteins c-myc - analysis
Random Allocation
Tumors
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Summary:Dietary restriction (DR) started in middle age profoundly reduces the occurrence of lymphoma in C57BL/6 mice. Here, we report immunocellular and molecular changes associated with this mode of cancer prevention. Twelve-month-old male C57BL/6 mice were either fed a control diet or subjected to moderate DR (approximately 25% < control intake). DR significantly reduced lymphoma development (incidence at 25 months, 19% of 72 control mice versus 5% of 60 DR mice). Flow cytometry of splenocytes showed that DR increased the percentage of CD4+ and CD8+ cells. Lymphomatous spleens displayed varied labeling patterns and high percentages of cells in S phase. Splenocyte c-myc expression tended to increase with age in controls and was reduced by DR. Lymphopenia and markedly reduced nucleated cell yields from peripheral lymphoid tissues were induced by DR. Serum interleukin 6 levels increased with age and were quite high (> 2500 pg/ml) in several mice with lymphoma and other histopathological findings. DR attenuated this age-associated increase. Immunohistochemical studies of lymphomatous spleens showed the presence of interleukin 6 in monocytic appearing cells but not in lymphoma cells. These observations support the possibility that an age-associated interleukin 6 dysregulation is important in lymphomagenesis.
ISSN:0008-5472
1538-7445