Adherence of Candida to Cultured Vascular Endothelial Cells: Mechanisms of Attachment and Endothelial Cell Penetration

To elucidate the pathogenesis of hematogenous Candida infections, we developed an in vitro model of Candida adherence to and penetration of human endothelial cells. We enhanced or inhibited adherence in order to probe mechanisms of attachment. Adherence of Candida albicans showed a linear relation t...

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Bibliographic Details
Published in:The Journal of infectious diseases 1985-12, Vol.152 (6), p.1264-1274
Main Authors: Rotrosen, Daniel, Edwards, John E., Gibson, Thomas R., Moore, Jeffrey C., Cohen, Arthur H., Green, Ira
Format: Article
Language:English
Subjects:
Adhesiveness
Animals
Antifungal Agents - pharmacology
Antiserum
Aorta
Biological and medical sciences
Candida
Candida albicans
Candida albicans - pathogenicity
Candida albicans - physiology
Candida albicans - ultrastructure
cell adhesion
Cell walls
Cultured cells
Cytochalasins
Endothelial cells
Endothelium
Endothelium - microbiology
Endothelium - physiology
Epithelial cells
Female
Fundamental and applied biological sciences. Psychology
Humans
Immune Sera - pharmacology
Inoculum
Mannans
Microbiology
Microscopy, Electron, Scanning
Mycology
Original Articles
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Rabbits
Saccharomyces cerevisiae - pathogenicity
Saccharomyces cerevisiae - physiology
Spores, Fungal - physiology
Time Factors
Umbilical Veins
vascular system
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Description
Summary:To elucidate the pathogenesis of hematogenous Candida infections, we developed an in vitro model of Candida adherence to and penetration of human endothelial cells. We enhanced or inhibited adherence in order to probe mechanisms of attachment. Adherence of Candida albicans showed a linear relation to Candida inoculum (range, 102–105 cfu, r = .99, P95% inhibition; P < .001), however, this activity was abolished by immunoprecipitation of immune serum with C albicans mannan (P 99% inhibition; P72% inhibition; P < .01). An extract from heat-exposed C albicans blocked adherence (>51% inhibition; P < .001). Transmission electron microscopy demonstrated that viable or killed Candida organisms were attached to endothelial cells, were enveloped by membrane processes from the endothelial cell surface, and were incorporated into the endothelial cells within phagosomes. Cytochalasin B blocked incorporation without blocking surface attachment.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/152.6.1264