Alterations in NFκB/Rel family proteins in splenic T-cells from tumor-bearing mice and reversal following therapy

• Published in: Cancer research (Chicago, Ill.) Ill.), 1994-06, Vol.54 (11), p.2969-2972
• Main Authors: PARITOSH GHOSH, SICA, A, YOUNG, H. A, JIANPING YE, FRANCO, J. L, WILTROUT, R. H, LONGO, D. L, RICE, N. R, KOMSCHLIES, K. L
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic agents; Antineoplastic Agents - therapeutic use; Base Sequence; Biological and medical sciences; Blotting, Western; Carcinoma, Renal Cell - chemistry; Carcinoma, Renal Cell - therapy; Cell Nucleus - chemistry; Chemotherapy; Electrophoresis; Flavonoids - therapeutic use; Interferon-gamma - analysis; Interleukin-2 - therapeutic use; Kidney Neoplasms - chemistry; Kidney Neoplasms - therapy; Male; Medical sciences; Mice; Mice, Inbred BALB C; Molecular Sequence Data; NF-kappa B - analysis; Pharmacology. Drug treatments; RNA, Messenger - analysis; T-Lymphocytes - chemistry
• ISSN: 0008-5472; 1538-7445

It has recently been shown that T-cell signal transduction molecules are altered in tumor-bearing mice. We have examined the expression of NF kappa B/Rel family proteins in T-cells from mice bearing Renca, a murine renal carcinoma. T-cells from Renca-bearing mice expressed undetectable levels of nuclear c-Rel, NF kappa B p65, and p50; however, two shorter forms of p50 (p48 and p46), truncated at the NH2-terminus, were present exclusively in the nucleus and were able to bind DNA. These T-cells have reduced expression of gamma-interferon mRNA. In mice successfully treated with flavone 8-acetic acid and recombinant human interleukin 2, the T-cells expressed normal levels of all three nuclear NF kappa B/Rel proteins. These results suggest that alterations in transcription factors may accompany changes in signal transduction molecules in T-cells from tumor-bearing animals; however, the changes are reversed with successful biological therapy.