Artificial induction of the acrosome reaction in human spermatozoa

This study investigated the use of human follicular fluid and pentoxifylline as inducers of the human sperm acrosome reaction in vitro. Motile sperm suspensions were prepared using a discontinuous Percoll gradient, preincubated for 3 h, divided into aliquots and exposed to various concentrations of...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1994-01, Vol.9 (1), p.77-82
Main Authors: Gearon, Ceinwen M., Mortimer, David, Chapman, Michael G., Forman, Robert G.
Format: Article
Language:English
Subjects:
Acrosome - drug effects
acrosome reaction
Arachis
Biological and medical sciences
Female
FITC-PNA
Fluorescein-5-isothiocyanate
follicular fluid
Follicular Fluid - physiology
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Lectins
Male
Mammalian male genital system
Morphology. Physiology
Peanut Agglutinin
pentoxifylline
Pentoxifylline - pharmacology
Plant Lectins
spermatozoa
Staining and Labeling
Vertebrates: reproduction
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Summary:This study investigated the use of human follicular fluid and pentoxifylline as inducers of the human sperm acrosome reaction in vitro. Motile sperm suspensions were prepared using a discontinuous Percoll gradient, preincubated for 3 h, divided into aliquots and exposed to various concentrations of non-heart-inactivated follicular fluid for 1 and 24 h and pentoxifylline for 30 min. Detection of the acrosome reaction involved the combined use of a fluorescent vital stain, H33258, and fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA). A short (1 h) exposure to follicular fluid at concentrations of 50% or more, did not compromise sperm motility and significantly increased the proportion of spermatozoa having completed the acrosome reaction. Similarly, a 30 min exposure to pentoxifylline also significantly increased the proportion of spermatozoa having completed the acrosome reaction.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a138324