Zona-opening of hamster oocytes: a comparative study using macro- and micro-manipulation methods

The present study compared a new macro-manipulation technique for zona-opening of hamster oocytes with existing micro-manipulation techniques. In experiment I the zona pellucida of hamster oocytes was partially opened with a 32 gauge steel needle (macro-manipulation) and these oocytes were then co-i...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1994-01, Vol.9 (1), p.137-140
Main Authors: Zhang, John, Liu, Jian, Tchabo, Jean G., Yang, Xiangzhong, DiMattina, Michael
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
Cricetinae
Female
Fertilization in Vitro
Gynecology. Andrology. Obstetrics
macro-manipulation
Medical sciences
Mesocricetus
micro-manipulation
Micromanipulation
Oocytes
Specimen Handling - methods
Sterility. Assisted procreation
Zona Pellucida
zona-opening
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Summary:The present study compared a new macro-manipulation technique for zona-opening of hamster oocytes with existing micro-manipulation techniques. In experiment I the zona pellucida of hamster oocytes was partially opened with a 32 gauge steel needle (macro-manipulation) and these oocytes were then co-incubated with human spermatozoa at 36.5°C in 5% CO2 in air for 3.5 h. Zona-free and zona-intact oocytes similarly inseminated served as controls. Of 113 oocytes, 30 (26%) lysed following zona-opening with the macro technique. The sperm penetration rates of zona-opened and zona-free oocytes were 37% (31/83) and 60% (49/81) respectively (P0.05). These results demonstrate that macro-manipulation results in similar penetration and polyspermia rates as compared to conventional micro-manipulation.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a138303